Source: International Congress 2014 – Cell biology 2014
Disease area: Airway diseases

Abstract

Lung fibroblasts are one of the most numerous mesenchymal cells, located directly beneath the epithelia. Fibroblasts secrete the extracellular matrix and provide the structural means for immune cells to move within the tissue. However there is now a growing appreciation of their ability to influence immune cells during inflammation. Successful interaction with T cells typically involves expression of the molecules of the immune synapse as well as uptake of exogenous material into vesicles. Human lung fibroblast were grown out from explants of resected tissue and were exposed to varying levels of IFN-g (0-1000U) and the expression of immune synapse molecules HLA-DR/DP/DQ, ICAM-1 and CD80/86 measured via flow cytometry at 48 hours. Fibroblasts were shown to express HLA and ICAM-1, with HLA increasing 6-foldb (N=10, P<0.0001) and ICAM-1 3-fold(N=11,P<0.005) following exposure to IFN-g. CD80/86 were not initially expressed and were not upregulated by IFN-g. Co-culture assays further demonstrated that activated T cells (5ug PHA, 48 hours) have a greater adherence to lung fibroblasts than inactive T cells and stimulating the fibroblasts with IFN-g further increases adhesion. Blocking experiments with anti-ICAM-1 antibody reduced adhesion. Using a combination of flow cytometry and confocal microscopy, fibroblasts were shown to internalize exogenous particles into vesicles. The current study highlights that lung fibroblasts have the potential to interact with immune cells, via immune synapse molecules HLA and ICAM-1, promote T cell adhesion within tissues, and have the capability to internalize environmental proteins, a trait of antigen presenting cells.

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Citations should be made in the following way:
A. Hutton, T. Wilkinson, J. Warner (Southampton, United Kingdom). LSC 2014 abstract - The ability of lung parenchymal fibroblasts to interact with T cells. Eur Respir J 2014; 44: Suppl. 58, 3857

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