Source: International Congress 2014 – Cell biology 2014
Disease area: Airway diseases

Abstract

Inhaled nanoparticles deposit on the airway epithelium. We are interested in how the effects of nanoparticles on the airway epithelium are changed after surface modification regarding impairment of mucociliary clearance and epithelial cytotoxicity.We tested the following 14-nm Carbon Black nanoparticles (CBNP) with similar hydrodynamic diameter: unmodified CBNP (CBNP/-), surface-modified CBNP with 9-nitroanthracene (CBNP/Na) or benzo[a]pyrene (CBNP/BaP) and acetylene soot (A. soot). After incubation of mouse tracheae with 10 or 30 µg/ml CBNP for 24 h, their effect on cilia-driven particle transport, apoptosis and necrosis, epithelial integrity and the mRNA expression of cytokines, mucins and cytochrome oxidases (Cyp) were determined.CBNP/- attached to cilia inducing an increase of ciliary beat frequency (CBF) and mucus release. In areas with mucus, particle transport speed (PTS) was decreased but in areas without mucus, PTS was increased. The epithelium remained intact and mRNA expression of cytokines, mucins or Cyp was not increased.A. soot also led to an increase of CBF, whereas CBNP/Na and CBNP/BaP did not alter CBF. But all three modified particles caused a decrease of PTS based on induction of apoptosis and cell membrane perforation. Although epithelial cells were lost, the remaining epithelial cells preserved epithelial integrity. In addition, CBNP/Na, CBNP/BaP and A. soot induced mRNA expression of Cyp1a1 and Cyp1b1, but A. soot alone increased mRNA expression of MIP-2, KC, IL-6 and Muc5ac. None of the modified CBNP induced mucus release.Our results indicate that the acute toxicity of CBNP is determined by their surface modification and modified CBNP can impair mucociliary clearance.

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Citations should be made in the following way:
K. Weinhold, P. König (Lübeck, Germany). The cytotoxicity of carbon black nanoparticles depends on their surface modification. Eur Respir J 2014; 44: Suppl. 58, 3856

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