Source: International Congress 2014 – Cell biology 2014
Disease area: Airway diseases, Respiratory infections

Abstract

Introduction and Objectives: Acute viral infections are common in healthy individuals without long-term health implications, but these infections can have more serious outcomes for infants, the elderly and immunocompromised individuals. Patients with chronic respiratory conditions, such as COPD, are at greater risk from severe adverse effects of acute respiratory infection. Investigation of how pulmonary immune cells of healthy and COPD patients differ in their responses to viral infection may elucidate defective mechanisms of host defense and allow for intervention to aid disease resolution.Methods: Human lung tissue from control or COPD patients undergoing lung resection surgery was infected with H3N2 strain X31 Influenza A virus (3V Biosciences, CA) or UV-irradiated X31 virus. Tissue was digested using collagenase to generate a single-cell suspension and was labelled with fluorescent antibodies for FACS analysis. The expression of the activation and exhaustion marker PD-1 was analysed on T cells, with the ligand PD-L1 measured on epithelial cells and macrophagess.Results: Proportion of CD4 T cells (mean=3.9%) and CD8 T cells (mean=10.9%) expressing PD-1 is greater in COPD individuals at baseline levels compared to controls (CD4 mean=1.2%, CD8 mean=2.4%). This baseline difference impacts upon PD-1 upregulation in response to X31 infection. Macrophages upregulate PD-L1 in response to infection in both COPD (1.2% to 6.6%, p=0.03) and control (1.3% to 16.6%, p=0.03) samples.Conclusion: Macrophages may play a role in controlling T cell responses to acute viral infection. This effect may be lost with high levels of PD-1 expression in COPD patients independently of X31 infection.

Rating:

Share or cite this content

Citations should be made in the following way:
R. McKendry, K. Staples, C. M. Spalluto, T. Wilkinson (Southampton, United Kingdom). Modulation of PD-1 and PD-L1 expression in response to acute viral infection of human lung tissue. Eur Respir J 2014; 44: Suppl. 58, 3855

Member's Comments

No comment yet.

Related content which might interest you: Induction of galectin-9 production by viral infection in the lung Source: International Congress 2015 – The multiple faces of host defense Year: 2015 Expression of CX3CL1 and CX3CR1 in human asthmatic lung in relation to cell survival Source: International Congress 2014 – New mechanisms in the pathogenesis of asthma and other lung diseases Year: 2014 Mapping the cellular source and role of IL-22 in murine lung infections Source: International Congress 2015 – Viral infections: basic research and clinical perspective Year: 2015 Hyperoxia impairs the inflammatory response of alveolar macrophages to multiple Toll-like receptor ligands associated with bacterial infection Source: International Congress 2014 – New insights into monocyte and macrophage biology Year: 2014 RSV infection modulates IL-15 production and MICA levels in respiratory epithelial cells Source: Eur Respir J 2012; 39: 712-720 Year: 2012 Crosstalk between lung fibroblasts and T-lymphocytes: Implications for anti-viral responses in chronic lung disease Source: Annual Congress 2013 –Asthma: new mechanisms and markers Year: 2013 Functional phenotype of immune response and alveolar macrophages phenotype in pulmonary sarcoidosis Source: International Congress 2014 – New insights into monocyte and macrophage biology Year: 2014 TNF-a induces cPLA2 expression via Jak2/PDGFR-dependent Elk-1/p300 activation in human lung epithelial cells Source: International Congress 2014 – Mechanistic studies of the airway epithelium Year: 2014 Differential sensitivity of subsets of monocyte-derived macrophages to apoptosis; its impact on lung inflammation Source: International Congress 2014 – New insights into monocyte and macrophage biology Year: 2014 Lymphocyte senescence in COPD is associated with loss of glucocorticoid receptor expression by cytotoxic/ pro-inflammatory lymphocytes Source: International Congress 2014 – Effect of cigarette smoke on cell behaviour: cell biology, signalling and senescence Year: 2014 LATE-BREAKING ABSTRACT: Inhibition of Pim1 kinase augments the anti-viral response to rhinovirus infection. Source: International Congress 2014 – COPD exacerbations Year: 2014 Modulation of neutrophil functionalities by indacaterol and their relevance for chronic lung diseases Source: International Congress 2014 – Translational studies in COPD Year: 2014 Interleukin-22 downregulates double-stranded RNA-induced expression of B7-H1/PD-L1 on airway epithelial cells Source: International Congress 2014 – Mechanistic studies of the airway epithelium Year: 2014 Influenza infection of human lung macrophages increases PDL1 expression Source: Annual Congress 2013 –Viral infections Year: 2013 Role of the immunoregulatory microRNA miR-146a in the innate immune response to rhinovirus infection Source: Annual Congress 2013 –Viral infections: what is new? Year: 2013 A role for the WFDC protein, WAP2, in regulation of innate host defence in the lung Source: International Congress 2014 – New studies into pulmonary proteases and antiproteases Year: 2014 Regulation of dectin-1 in cystic fibrosis bronchial epithelial cells during host pathogen interactions Source: International Congress 2016 – Cystic fibrosis: inflammation, microbiology, management and monitoring Year: 2016 Imbalance of immune response functional phenotype and alveolar macrophages phenotype in COPD Source: International Congress 2014 – New insights into monocyte and macrophage biology Year: 2014 The role of chemokine receptors in the aberrant migration of COPD neutrophils Source: International Congress 2014 – Cell biology 2014 Year: 2014 LSC 2014 abstract - Targeting of microRNAs can restore innate anti-viral response in asthma Source: International Congress 2014 – Novel epigenetic regulators for targeting via nanomedicine Year: 2014