Source: International Congress 2014 – Cell biology 2014
Disease area: Airway diseases

Abstract

The role of cadmium with respect to cell growth is still under debate. Low concentration of cadmium was applied to mice primary lung epithelial cells and cell proliferation was measured both by cell cycle analysis and Brdu incorporation assay, effects of different dose of cadmium on lung epithelial cells was evaluated morphologically by atomic force microscopy. RT-PCR and western blot confirmed the specific signalling pathways regarding cadmium induced lung cell proliferation. Primary lung epithelial cells were transfected with siEGFR and molecular interactions of downstream signalling molecules were determined. We have reported RNAi induced silencing of the EGFR, which is over expressed in cadmium induced primary mouse lung epithelial cells. siEGFR effectively prevents expression of proinflammatory and cell proliferative markers. Moreover N-acetyl cysteine mediated inhibition of EGFR and downstream signalling molecules indicate the involvement of reactive oxygen species induced up regulation of EGFR by lower dose of cadmium. We have evaluated how exposure to low concentration of cadmium promotes the growth of primary mice lung epithelial cell by EGFR signalling. We have also transfected the primary lung epithelial cell with siRNA against the regulatory subunit of nuclear factor kb (NF-kb) and the data shows that cadmium induced lung cell proliferation is the effect of EGFR mediated NF-kb activation.



Rating:

Share or cite this content

Citations should be made in the following way:
A. Bhattacharyya (Kolkata, India). NF-κB-EGFR cross talk in lung cell proliferation. Eur Respir J 2014; 44: Suppl. 58, 3852

Member's Comments

No comment yet.

Related content which might interest you: Adaptation of lung epithelial cells subjected to chronic cyclic strain Source: International Congress 2014 – Mechanistic studies of the airway epithelium Year: 2014 Carbocysteine reverts the effects of cigarette smoke on the growth and on the senescence of bronchial epithelial cells Source: International Congress 2014 – Effect of cigarette smoke on cell behaviour: cell biology, signalling and senescence Year: 2014 The cytotoxicity of carbon black nanoparticles depends on their surface modification Source: International Congress 2014 – Cell biology 2014 Year: 2014 Cell intrinsic and environmental factors influencing human bronchial epithelial cell migration Source: International Congress 2016 – Lung cancer and immune cells: friends or foes? Year: 2016 Reconstituted human airway epithelium 3D-model to assess the impact of air pollutants on the inflammatory response Source: International Congress 2014 – Mechanistic studies of the airway epithelium Year: 2014 Investigation of the mechanism(s) of oxygen-induced ciliated epithelial loss in bovine bronchus tissue Source: International Congress 2014 – Mechanistic studies of the airway epithelium Year: 2014 Complex extracellular matrix modulates neutrophil migration in vitro Source: International Congress 2014 – New mechanisms in the pathogenesis of asthma and other lung diseases Year: 2014 Crosstalk between epithelium and fibroblasts; implications for COPD Source: International Congress 2014 – Mechanistic studies of the airway epithelium Year: 2014 S100 proteins induce MUC5AC production in airway epithelial cells Source: International Congress 2014 – Mechanistic studies of the airway epithelium Year: 2014 TNF-a induces cPLA2 expression via Jak2/PDGFR-dependent Elk-1/p300 activation in human lung epithelial cells Source: International Congress 2014 – Mechanistic studies of the airway epithelium Year: 2014 Transcriptional analysis of the human BPIFA1/SPLUNC1 gene Source: Annual Congress 2013 –Stress responses and T-cell behaviour in the lung Year: 2013 Comprehensive characterization of the lung proteasome Source: International Congress 2014 – Cell biology 2014 Year: 2014 Mitochondrial function in three different types of airway epithelial cells Source: International Congress 2015 – Translational studies in airway cell biology Year: 2015 Imaging of epithelial repair processes in the mouse trachea after laser-induced injury Source: International Congress 2014 – Mechanistic studies of the airway epithelium Year: 2014 Aspiration, epithelial injury and lung diseases Source: International Congress 2015 – New insights into lung disease pathogenesis Year: 2015 Human lung mast cells alter asthmatic airway epithelial cell function Source: Annual Congress 2013 –The pulmonary epithelium: recent developments Year: 2013 Cigarette smoke affects differentiation and function of primary human bronchial epithelial cells Source: International Congress 2014 – Effect of cigarette smoke on cell behaviour: cell biology, signalling and senescence Year: 2014 The effects of INTS12 knockdown on processing of snRNAs Source: International Congress 2014 – Novel epigenetic regulators for targeting via nanomedicine Year: 2014 Different roles for PI3Kgamma and δ in asthma Source: International Congress 2014 – Animal models: asthma, COPD and beyond Year: 2014 A potential role for endothelial adhesion molecules and cytokines in chemotherapy-induced lung fibrosis Source: International Congress 2014 – Cell biology 2014 Year: 2014