Source: International Congress 2014 – Cell biology 2014
Disease area: Airway diseases

Abstract

Asthma is characterized by variable airflow obstruction, airway inflammation, airway hyper-responsiveness and airway remodeling. Airway smooth muscle hyperplasia is a feature of airway remodeling and contributes to bronchial wall thickening.We sought to investigate the expression levels of chemokines in primary cultures of airway smooth muscle cells from asthmatics and healthy controls and to assess whether differentially expressed chemokines a)promote fibrocyte migration and b)are increased in blood from subjects with asthma and in sputum samples from those asthmatics with bronchial wall thickening.Chemokines released by healthy and asthmatic primary airway smooth muscle were measured by MesoScale Discovery platform and the chemokine most highly expressed was confirmed by ELISA. Expression of its cognate chemokine receptor by fibrocytes was examined by immunofluorescence and flow cytometry. The role of this chemokine in fibrocyte migration towards airway smooth muscle was investigated by chemotaxis assays.CCL2 levels were elevated in primary airway smooth muscle supernatants from asthmatics compared to healthy controls (1880±329 versus 947±203 pg/10⁶ cells,p=0.02). CCR2 was expressed on fibrocytes. Fibrocytes migrated towards recombinant CCL2 and airway smooth muscle supernatants. These effects were inhibited by CCL2 neutralisation. CCL2 levels were increased in blood from asthmatics compared to healthy controls (136±14 versus 78±11 pg/mL,p=0.002) and sputum CCL2 was elevated in asthmatics with bronchial wall thickening (p=0.008).Airway smooth muscle-derived CCL2 mediates fibrocyte migration and potentially contributes to the development of airway smooth muscle hyperplasia in asthma.

Rating:

Share or cite this content

Citations should be made in the following way:
S. Singh, A. Sutcliffe, D. Kaur, S. Gupta, D. Desai, R. Saunders, C. Brightling (Leicester, United Kingdom). CCL2 release by airway smooth muscle is increased in asthma and promotes fibrocyte migration. Eur Respir J 2014; 44: Suppl. 58, 3848

Member's Comments

No comment yet.

Related content which might interest you: MNK-1 inhibition reduces proliferation and CXCL10 in airway smooth muscle cells Source: Annual Congress 2013 –Airway smooth muscle and cell biology Year: 2013 Corticosteroid insensitivity in airway smooth muscle cells from severe asthma is dependent on stimulus and cytokine product Source: International Congress 2014 – Cell biology 2014 Year: 2014 Integrins regulate eosinophil adhesion to the airway smooth muscle cells in asthma patients Source: International Congress 2015 – Asthma mechanisms and management Year: 2015 Airway smooth muscle contractility is increased following coculture with fibrocytes Source: International Congress 2016 – Mechanisms of disease Year: 2016 The effect of JQ1/SGCBD01 upon c-Myc in human airway smooth muscle in asthma Source: International Congress 2014 – New treatments for cough, asthma, COPD and ILDs Year: 2014 Eosinophil-induced airway smooth muscle cells remodeling is attenuated by blocking of RGD-binding integrins in asthma Source: International Congress 2016 – New findings in mucosal immunology Year: 2016 Link between airway inflammation and remodeling: TNFα induces airway smooth muscle cell proliferation via an ET1/GMCSF/IL6 network Source: International Congress 2015 – Airway remodelling: recent developments Year: 2015 DP2/CRTh2 is expressed by ASM cells in asthma and its inhibition suppresses ASM migration Source: International Congress 2016 – Mechanisms of disease Year: 2016 HMGB1 is upregulated in ASM in asthma and induces ASM contraction via TLR4 Source: International Congress 2015 – Immunomodulation: basic science and clinical aspects Year: 2015 Hypoxia exerts dualistic effects on inflammatory and proliferative responses of healthy and asthmatic primary human bronchial smooth muscle cells Source: International Congress 2014 – Cell biology 2014 Year: 2014 Secretion of hyaluronic acid is mediated by muscarinic receptors in airway smooth muscle cells of patients with COPD Source: International Congress 2014 – Translational studies in COPD Year: 2014 Extracellular matrix within the airway smooth muscle layer correlates with age but not in cases of asthma Source: International Congress 2016 – Translational studies in lung disease Year: 2016 The role of glycolysis in TGF-beta-mediated airway smooth muscle cell proliferation Source: International Congress 2014 – Cell biology 2014 Year: 2014 Targeting ASK1 in preventing airway smooth muscle growth: Implications for airway remodeling in COPD Source: International Congress 2016 – New findings in mucosal immunology Year: 2016 A functional role for WNT-5A in driving airway myocyte proliferation Source: Annual Congress 2013 –Growth factors at the crossroads of acute and chronic inflammation Year: 2013 Allergic asthma induce opposite changes in the expression of caveolin-1 and cavin-2 in airway and vascular pulmonary smooth muscle Source: International Congress 2014 – Animal models: asthma, COPD and beyond Year: 2014 The effect of adenosine on VEGF release from airway smooth muscle from subjects with asthma Source: Annual Congress 2013 –Airway smooth muscle and cell biology Year: 2013 Plasminogen-stimulated inflammatory cytokine production by airway smooth muscle cells is regulated by annexin A2 Source: Annual Congress 2013 –Airway smooth muscle and cell biology Year: 2013 Distribution of increased airway smooth muscle thickness and airway inflammation in asthma Source: Annual Congress 2013 –Airway smooth muscle and cell biology Year: 2013 Mitochondrial membrane potential in the airway and skeletal muscle compartments of smokers with and without COPD Source: International Congress 2014 – Translational studies in COPD Year: 2014