Source: Annual Congress 2002 - Cell biology and genetics of COPD and emphysema
Disease area: Airway diseases, Pulmonary vascular diseases

Abstract

The aim of the study was to evaluate the influence of heparin on permeability of cutaneous and mesenteric microvessel endothelium of rats with hypobaric hypoxia (HH) and hypertension-induced hypoxia (HIH).
Induction of chronic HH in rats (n=65) was performed in a pressure chamber in a course of 25 sessions (one session per day), the atmospheric pressure being gradually decreased with each session from 57.7*10(3) Pa to 47.2*10(3) Pa by the end of the course. The current report presents the findings at day 10 of hypoxia. Renovascular arterial hypertension in rats (n=48) was induced by H. Goldblatt (1934) method, evaluation of permeability was performed 6 months after the operation when the stable mean level of hypertension leading to hypoxia has been achieved (BP: 163±]4 mm Hg). The permeability of microvessel endothelium was assessed by contact fluorescent biomicroscopy with Na-fluorescein (0.25 mg/100 g body weight). Role of heparin in permeability was evaluated in animals, chronically heparinized by a continuous daily injection of 0.01 units per 100 g body weight of non-fractional heparin for 7 days measurement.
Permeability of endothelium both in skin (11.0±]0.9 units) and mesentery (4.3±]0.9 units) of heparinized animals was found to be significantly lower than in non-heparinized rats with HH (23.6±]1.1 units, p<0.001; 9.7±]0.7 units, p<0.01, resp.), the latter being significantly increased as compared to intact controls. In rats with HIH the permeability was also significantly increased (22.0±]1.8 units, p<0.01) compared to intacts, and the heparinized animals revealed twice as low permeation rate (p<0.01) as the one in non-heparinized animals.
We conclude that heparin decreases endothelial permeability in cutaneous and mesenteric microvessels in both types of hypoxia.

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Citations should be made in the following way:
N. A. Gavrisheva, M. V. Dubina, A. V. Fedoulov (St-Petersburg, Russia). Heparin decreases permeability of microvascular endothelium in rats with hypobaric and hypertension-induced chronic hypoxia. Eur Respir J 2002; 20: Suppl. 38, 644

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