Source: Annual Congress 2002 - Cell biology and genetics of COPD and emphysema
Disease area: Airway diseases

Abstract

The target of investigation is to establish effector cell types and its apoptotic index (AI) in the induced sputum of patients with COB. 29 patients were examined in the acute condition , 31 ones - in the remission period, 20 people were control.
The typing of cells was performed by immune-fluorescent method with help of monoclonal antibodies. To reveal apoptotically changed cells the dyestuff Hoechst 33258 was used. Against background of increased containment of cells totally in 1 ml - 3.2 + 0.6 - we revealed the increased level of neutrophils carrying CD-32 anti-gene to 56.3 + 3.1 %, CD-8 lymphocytes to 33.2+2.3 %, insignificant increasing of CD HLA-DR to 9.6+ 0.9%, CD -11 eosinophils to 6.8+0.6 %. We marked low index of AI in nucleus of all the cells, taking part in inflammation in different periods of disease. See the data in table below:

	CD-11	HC-DR	CD-8	CD-32	CD-4	CD-20Lymph
control	78.1+0.9	78.6+0.8	72.1+1.1	78+0.7	76.4+1	74.3+1.4
COB -acute	26.7+1.2	30+2.1	29.4+1.6	31.8+1.8	24+2	22.3+0.8
COB-remission	39.4+0.9	42.4+1.5	60.1+0.4	63.6+1.1	41.6+1.4	39.4+0.6

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Citations should be made in the following way:
V. A. Nevzorova, S. A. Pazych (Vladivostok, Russia). The typing of cells in the induced sputum (IS) and its apoptosis in patients with chronic obstructive bronchitis (COB). Eur Respir J 2002; 20: Suppl. 38, 642

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