Source: Annual Congress 2002 - Cell biology and genetics of COPD and emphysema
Disease area: Airway diseases

Abstract

The diaphragm is a complex muscle designed to work for life and capable to generate shortening. It encounters many traumatic events such as cough or increased airways resistance which result in damage of its fibers. As we tested in an alive rat model, the repair of injured fibers is essentially completed in 10 days while the muscle continues to work. We establish that 3 structural and functional phases characterize diaphragmatic fibers recovery from damage. Phase 1- Injury, sarcomeric and membrane debris are removed, and the membrane is sealed. This phase (# 4 days) is charactrized by an increase of the expression of the muscle-specific calpains (1h), myogenin (starting at 12 hours) and the activation of macrophages, while force generation is highly reduced. Phase 2- Structure rebuilding i.e. re-synthesis of the damaged proteins, reconstruction of the sarcomeres and different organelles (4-8days). It is characterized by an increased expression of bFGF, IGF II, c-Fos, a decline of myogenin and subsequently a rise in myosin and titin expression. This phase is essential to regulate the proliferation and the differentiation of the myofibers. Phase 3- Recovery of function where innervation is regained and satellite cells cease to divide. It is completed in about 10 days when fibers are repaired, their innervation and circulation completed, and full physiological properties recovered.

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Citations should be made in the following way:
S. N. Mehiri, G. Czaika, M. Hayot, A. Grassino (Montréal, Canada; Montpellier, France). Chronology of diaphragm repair. Eur Respir J 2002; 20: Suppl. 38, 641

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