Source: Annual Congress 2002 - Cell biology and genetics of COPD and emphysema
Disease area: Airway diseases

Abstract

Mucociliary transport is dependant on cilia which line the walls of the airways. The frog palate model has been used to study mucociliary transport (MT) under normal conditions. We have extended the use of this model by creating an injury to the palate with sodium metabisulphite (MB). Studies were undertaken to characterize the nature of the induced injury. MT was measured following the application of MB to the palate in increasing concentrations (10^-4, 10^-2, 10^-1 M). MB 10^-1 M produced a 293 ±] 122% increase in MT expressed as % control, within 2min of topical application (2-3μl) to the palate. Ciliary beat frequency (CBF) was measured by digital video (60 frames/sec) in a separate control palate at 8 ±] 1 beats/sec. Ciliary activity in MB-treated palates was not discernable 2min after topical application. Scanning electron microscope (SEM) studies of the ciliated epithelial surface of the palate showed that intact ciliated cells were lost from the palate surface after MB. Intact ciliated cells were found in mucus collected from the palates. Increased matrix metalloproteinase activity (MMP2) measured by zymography was seen in MB-treated vs. control palates. We conclude that MB exerts an acute effect on MT by inhibiting CBF. A more sustained effect, which interfered with the recovery of normal MT was generated by the loss of ciliated epithelial cells. The mechanism of action of this effect may involve activation of MMPs by MB and suggests that inflammatory mediators may contribute to airway remodeling and impaired mucociliary transport by activating MMPs.

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D. W. O'Brien, M. I. Morris, Y. Xu, M. King (Edmonton, Canada). Sodium metabisulphite in a frog palate injury model activates matrix metalloproteinases leading to loss of cilia and impaired mucociliary transport. Eur Respir J 2002; 20: Suppl. 38, 636

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