Source: International Congress 2014 – Pathology and prognostic factors of thoracic tumours
Disease area: Thoracic oncology

Abstract

Introduction: Several treatments in non-small cell lung cancer (NSCLC) are histology-dependent, and the need for histology-related markers is increasing. We have examined the prognostic value of the miR-200 family in overall survival (OS) of early-stage NSCLC patients with adenocarcinoma or squamous cell carcinoma (SCC).Experimental design: The expression of all five members of the miR-200 family was determined using TaqMan assays in 155 tumors from resected NSCLC patients. Functional characterization studies were conducted in the H23 lung cancer cell line.Results: High miR-200c expression was associated with shorter OS in the entire cohort (P = 0.024). High miR-200c (P = 0.0004) or miR-141 (P = 0.009) expression correlated with shorter OS in adenocarcinoma – but not in SCC.In the multivariate analysis, the miR-141/200c score emerged as an independent prognostic factor for OS in the entire cohort (OR, 2.787; P = 0.033) and in adenocarcinoma patients (OR, 10.649; P = 0.002) but not in SCC patients. Functional analyses showed that miR-200c, but not miR-141, was related to mesenchymal-epithelial transition (MET) and affected cell migration and E-cadherin levels, while overexpression of miR-141 was associated with higher levels of VEGF in vitro (P = 0.04) and with higher blood microvessel density in patient tumor samples (P < 0.001).Conclusions: High miR-141 and miR-200c expression are associated with poorer outcome in NSCLC patients with adenocarcinoma but not in those with SCC. miR-141 seems to act through angiogenesis, while miR-200c may play a role in the regulation of MET.FIS 12/405, SEPAR, SOCAP, FUCAP.

Rating:

Share or cite this content

Citations should be made in the following way:
J. Moisés, R. Marrades, R. Tejero, A. Navarro, M. Campayo, N. Viñolas, C. Agustí, A. Saco, M. Paradela, L. Molins, J. Ramirez, M. Monzó (Barcelona, Spain). Prognostic value and functional characterization of miR-141 and miR-200c expression levels in resected NSCL. Eur Respir J 2014; 44: Suppl. 58, 513

Member's Comments

No comment yet.

Related content which might interest you: The value of miR221 and miR222 as a biomarker in non small cell lung cancer Source: International Congress 2014 – Pathology and prognostic factors of thoracic tumours Year: 2014 Differences in expression and prognostic values of p16(ink) and VEGF in squamous and adenocarcinoma of the lung Source: International Congress 2014 – Pathology and prognostic factors of thoracic tumours Year: 2014 Significance of EGFR expression patterns in patients with non-small cell lung cancer – Evaluation on protein- and/or gene level? Source: International Congress 2014 – Pathology and prognostic factors of thoracic tumours Year: 2014 Expression analysis of miR-21 in Bulgarian patients with non-small cell lung cancer Source: International Congress 2016 – Prognostic variables in lung cancer I Year: 2016 beta-F1-ATPase expression as a prognostic factor for resected non-small cell lung cancer Source: Annual Congress 2013 –Prognostic factors and subtyping of lung cancer Year: 2013 Investigation of survivin gene expression in non small cell lung cancer Source: International Congress 2014 – Biology and pathology of thoracic tumours Year: 2014 Micro-RNA screening in neuroendocrine lung tumors: Differential expression of miR-29 family members Source: Annual Congress 2013 –New strategies in epigenomic research to study lung diseases (DNA rearrangements, RNA, methylation, proteosome) Year: 2013 Prognostic value of survivin gene promoter polymorphisms in non-small cell lung cancer Source: International Congress 2016 – Prognostic variables in lung cancer I Year: 2016 Serum IL-33 as a diagnostic and prognostic marker in non- small cell lung cancer Source: International Congress 2014 – Pathology and prognostic factors of thoracic tumours Year: 2014 Downregulated anti-angiogenic miR-19a, miR-126 and let-7b in non-small lung cancer have poor but different prognostic values in squamous and adenocarcinoma subtypes Source: Annual Congress 2013 –Biology and molecular pathology of lung cancer Year: 2013 Correlation between the simultaneus expression of HER2 and PTEN and the risk of development of metastasis in NSCLC Source: International Congress 2014 – Pathology and prognostic factors of thoracic tumours Year: 2014 Gene expression of EGFR, MINA53,MEN1 and MTOR in NSCLCs Source: International Congress 2014 – Biology and pathology of thoracic tumours Year: 2014 The prognostic impact of PD1 and PDL1 expression in non-small cell lung cancer Source: International Congress 2015 – Lung cancer: prognostic factors and features of oncogene-driven tumours Year: 2015 Proapoptotic effects of PAI-1 protein on lung and prostate cancer cells Source: Annual Congress 2013 –Basic research on lung cancer Year: 2013 Prognostic value of EGFR mutation in NSCLC in surgical stage Source: International Congress 2014 – Biology and pathology of thoracic tumours Year: 2014 Immunohistochemical expression of nuclear factor kappa-B/p65 and cyclooxygenase-2 in non-small cell lung cancer patients: Prognostic value and impact on survival Source: International Congress 2014 – Pathology and prognostic factors of thoracic tumours Year: 2014 Expression and clinical significance of S100 calcium binding protein A2 in lung cancer Source: International Congress 2016 – Prognostic variables in lung cancer II Year: 2016 Simultaneous EGFR and VEGF alterations in non-small cell lung carcinoma based on tissue microarrays Source: International Congress 2014 – Pathology and prognostic factors of thoracic tumours Year: 2014 Correlation of miR-422a, miR-21 and miR-155 analysis with hMSH2 and hMLH1 mRNA expression profiles in non-small cell lung carcinomas and their adjacent normal tissues Source: Annual Congress 2013 –Biology and molecular pathology of lung cancer Year: 2013 Polymorphism of HLA-G gene promotor in patients with non small cell lung cancer Source: International Congress 2014 – Biology and pathology of thoracic tumours Year: 2014