Source: International Congress 2014 – Occupational asthma: from childhood to adulthood
Disease area: Airway diseases

Abstract

Aim Upper airway reactions in subjects with work related asthma were investigated by proteomic analysis.Methods Altogether 195 subjects participated the study. Four groups (8 subjects/ group) were formed for 2D-DIGE analysis of nasal brush specimens: healthy controls, asthmatics with symptoms related to protein allergens, isocyanates or welding fumes. Unsupervised clustering (DeCyder 2-D Differential Analysis Software) and protein network (Ingenuity System's database) analyses were performed after gel spot protein identifications by MS/MS (nanoLC-QExactive).Results On average 2500 spots per DIGE gel were detected; 218 protein spots were picked for identification yielding 125 different proteins. Exposure to protein allergens had the largest effects on the proteome. Hierarchical clustering revealed that subjects with symptoms to protein allergens and isocyanates have similar profiles, whereas subjects exposed to welding fumes differed from these groups. The highly overrepresented functional categories were defense response, protease inhibitor activity, immunity, response to inflammation and wounding, complement activation and cellular response to oxidative stress. Substantial down-regulations of Annexin A1 and A2 proteoforms and oxidative stress defense proteins (GST, SOD and PRXs) are associated with the exposure to protein allergens and isocyanates.Conclusions Subjects exposed to protein allergens and isocyanates elicit similar nasal proteome responses, even though no IgE-mediated sensitization was detected to isosyanates. Identified changes in protein expressions reveal biological activities related to airway inflammation, oxidation-reduction, tissue matrix turnover and immunity.

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H. Suojalehto, I. Lindström, N. Ahonen, A. Puustinen (Helsinki, Finland). Proteomic analysis of the airway inflammation in work related asthma. Eur Respir J 2014; 44: Suppl. 58, 408

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