Differential expression profile of miRNAs and piRNAs in asthmatic and non-asthmatic bronchial smooth muscle cells reveals new possible biomarkers for complex lung diseases
N. Miglino, E. Alexandrova, A. Hashim, M. Ravo, M. Tamm, F. Baty, M. Brutsche, A. Weisz, P. Borger (Basel, St. Gallen, Switzerland; Salerno, Italy)
Source: International Congress 2014 – Novel approaches in transcriptomics and epigenomics in inflammatory lung diseases and lung cancer
Disease area: Airway diseases
Abstract Bronchial smooth muscle (BSM) cells are the effector cells of bronchoconstriction and a potent source of inflammatory mediators and angiogenic factors. Small non-coding (snc)RNAs, such as microRNAs (miRNAs) and PIWI-interacting RNAs (piRNAs) are transcriptional and post-transcriptional regulators of gene expression. Abbarently expressed, they may play a role in airway diseases and/or provide novel biomarkers to characterize lung pathologies.To assess the miRNAs and piRNAs expression profiles of primary human BSM cells of asthmatic (n=8) and non-asthmatic (n=6) individuals. Library preparation was performed with Illumina TruSeq small RNA sample preparation protocol. Sized smallRNA libraries were gel purified and sequenced on Illumina HiSeq 1500. iMir [BMC Bioinformatics, Dec 2013, 13; 14:362) was used to identify sncRNAs and perform differential expression analysis.BSM cells of both asthmatics and non-asthmatics expressed 892 and 122 known miRNAs and piRNAs, respectively. 33 miRNAs were differentially expressed in asthmatic vs non-asthmatic BSM cells (p£ 0.05, fold-change cut-off³ 1.5) and among them 27 were up-regulated and 6 down-regulated. Furthermore, 1 piRNA was found significantly down regulated in BSM cells from asthmatic patients.We conclude that BSM cells from both asthmatics and non-asthmatics are characterized by distinctly different pools of miRNAs and piRNAs. Differential expression of miRNAs and piRNAs may contribute to, and/or explain, the development of airway diseases and provide novel biomarkers to characterize and discriminate between lung pathologies at an early stage of the diseases.
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N. Miglino, E. Alexandrova, A. Hashim, M. Ravo, M. Tamm, F. Baty, M. Brutsche, A. Weisz, P. Borger (Basel, St. Gallen, Switzerland; Salerno, Italy). Differential expression profile of miRNAs and piRNAs in asthmatic and non-asthmatic bronchial smooth muscle cells reveals new possible biomarkers for complex lung diseases. Eur Respir J 2014; 44: Suppl. 58, 398
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