Source: Annual Congress 2002 - Cellular reactions in allergy in humans and animals
Disease area: Airway diseases

Abstract

Immunization with naked plasmid DNA leads to strong and persistent cell-mediated and humoral immune response to the antigen encoded by plasmid. Recently vaccines based on plasmid DNA encoding T cell epitopes on Der p induced immunoprotective effect on allergic response. However, little information is currently available on the therapeutic efficacy of DNA encoding the T cell epitope in the allergic disease. The purpose of this study was to evaluate whether the therapeutic effect of DNA vaccination with T cell epitope on allergic responses. To address this issue, on 4 weeks after immunization of Der p allergen extract, the vaccination group was injected with the mixed naked DNA plasmids encoding the murine T cell epitope on Der p 2, on three successive weeks into muscle of BALB/c mice. The control mice group was injected with the pcDNA 3.1 blank vector. On 9 weeks after immunization, all mice were challenged with the Der p extracts on 3 successive days. The vaccination group(1.96±]0.67 mg/ml) inhibited significantly the level of the Immunoglobulin E antibody response than the control mice group (5.96±]1.96 mg/ml). These result suggest that gene therapy using T-cell epitope encoding DNA could be an ideal way of combating allergic disease in future.

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S. S. Kwon, S. Y. Lee, S. J. Kim, Y. K. Kim, K. H. Kim, H. S. Moon, J. S. Song, S. H. Park, T. J. Yoo (Sechogu, South Korea; Memphis, United States Of America). The vaccination with DNA encoding T cell epitope on the Der p inhibited allergic responses in mice. Eur Respir J 2002; 20: Suppl. 38, 302

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