Source: Annual Congress 2002 - Cellular reactions in allergy in humans and animals
Disease area: Airway diseases

Abstract

Interleukin-5 (IL-5) plays a key role in the development of airway inflammation in asthma. IL-5 is produced mainly by T cells. To observe the association of the activation of T cells and expression of IL-5 mRNA, co-expression of CD25 and IL-5 mRNA were assessed with methods of immunocytochemistry and in situ hybridization double staining.
We found that there was a strong tendency to co-expression of IL-5 mRNA together with CD25 in T cells. 57.8+/-11.3% and 25.3+/-10.7% T cells from allergic mice were CD25(+)/IL-5 mRNA(+) and CD25(-)/IL-5 mRNA(-) respectively. CD25(-)/IL-5 mRNA(+) and CD25(+)/IL-5 mRNA(-) T cells were only 10.2+/-4.7% and 6.7+/-3.6% respectively. CD25(+)/IL-5 mRNA(+) T cells from allergic mice treated by dexamethasone decreased markedly (11.0+/-2.2% vs. 57.8+/-11.3%, p<0.01), and CD25(-)/IL-5 mRNA(-) T cells increased significantly (76.4+/-7.5% vs. 25.3+/-10.7% , p<0.01). These results indicate that expression of IL-5mRNA associate strongly with activation of T cells.

Rating:

Share or cite this content

Citations should be made in the following way:
C. Z. Wang, X. M. Cheng (Chongqing, China). T-cells from allergic mice co-expressing IL-5 mRNA together with CD25. Eur Respir J 2002; 20: Suppl. 38, 295

Member's Comments

No comment yet.

Related content which might interest you: Effects of CD4+CD25+ T cells on the production of IL-4, IL-5 and IFN-γ by CD4+ T cells in asthmatic mice Source: Eur Respir J 2005; 26: Suppl. 49, 24s Year: 2005 High-level expression of PD-L2 molecules by dendritic cells suppressed the Th2 response in allergic mouse Source: Eur Respir J 2007; 30: Suppl. 51, 368s Year: 2007 Overproduction of IL-18 in the lungs induces IL-13 and IFN-g producing CD4+ T cell in the lungs, and results in airway hyperresponsiveness in Balb/c mice Source: Annual Congress 2011 - Asthma: basic science and clinical studies Year: 2011 Enhanced expression of IL-18 and increased CD8+ T cells and eosinophils in the airways of asthma death Source: Annual Congress 2010 - Mechanisms of asthma and lung inflammation Year: 2010 IL-10 produced by CD4+ T cells is essential for the development of airway hyperresponsiveness via suppression of endogenous IL-12 Source: Eur Respir J 2005; 26: Suppl. 49, 24s Year: 2005 Allergen immunotherapy downregulate CD86 expression on dendritic cells and upregulate CTLA-4 expression on T cells in a mouse asthma model Source: Eur Respir J 2005; 26: Suppl. 49, 135s Year: 2005 Modified mRNA encoding Foxp3 protects against allergic asthma in mice by rebalancing T helper cell responses through an IL-23 / IL-17A-dependent mechanism Source: Annual Congress 2012 - Treating childhood asthma Year: 2012 Interleukin 4 (IL-4) mRNA expression in peripheral blood CD4+ T cells and adhesion molecule profile in patients with bronchial asthma Source: Eur Respir J 2001; 18: Suppl. 33, 272s Year: 2001 mRNA expression for Th2 and Th1 derived cytokines in induced sputum Source: Eur Respir J 2001; 18: Suppl. 33, 271s Year: 2001 High concentration of antigen induces allergic mice T cells anergy with depressing CD86 expression on antigen-presenting cells Source: Eur Respir J 2002; 20: Suppl. 38, 28s Year: 2002 The PBMC of severe asthmatics showed enhanced candida antigen induced IL-5, IL-13 and TARC production and this enhancement was inhibited by dendric cells deprition Source: Annual Congress 2009 - Inflammatory cells and cytokines Year: 2009 Upregulation of CD4+ and CD8+ cells expressing CX3CR1 in asthmatics Source: Annual Congress 2006 - Towards understanding the pathogenesis of asthma Year: 2006 Expression Of CD80 and CD86 on T lymphocytes and monocytes of asthmatic children Source: Eur Respir J 2003; 22: Suppl. 45, 400s Year: 2003 The effects of DC expressing PD-L1 on CD4+ T cell in a murine model of allergic airway disease Source: Annual Congress 2007 - Mouse models of allergic airway inflammation Year: 2007 IL-15 transpresentation in circulating progenitors of NK cells controls their differentiation potential, STAT6 activation and IL-13 production Source: Annual Congress 2005 - Regulation of the allergic and asthmatic response Year: 2005 ILC2 cells from severe allergic and eosinophilic asthma demonstrate increased expression of TSLP receptor (TSLPR) and enhanced proliferative capacity. Source: Virtual Congress 2021 – New insight into the immunology of allergies, asthma and COPD: from mouse to man Year: 2021 IL-9 and IL-9 receptor (IL-9r) expression in BALF lymphocytes in ILD patients: preliminary results. Source: International Congress 2017 – The wide spectrum of ILDs Year: 2017 Different Th1, Th2, Th17 and Treg associated chemokine local pattern expression in lung in a murine model of allergic inflammation Source: Annual Congress 2011 - T-cell subsets Year: 2011 Down regulation of ICOS and CTLA 4 on allergic asthmatic T cells upon Derp1 stimulation Source: Annual Congress 2006 - Novel mechanisms in asthmatic inflammation Year: 2006 Macrophages induce an allergen-specific and long-term suppression in a mouse asthma model Source: Eur Respir J 2005; 26: 1040-1046 Year: 2005