Development of allergic airway inflammation may be associated with prompt structural changes of small blood vessels
K. Rydell, L. Uller, A. Bengtsson, C. G. A. Persson, J. S. Erjefält (Lund, Sweden)
Source: Annual Congress 2002 - Cellular reactions in allergy in humans and animals
Session: Cellular reactions in allergy in humans and animals
Session type: Poster Discussion
Number: 293
Disease area: Airway diseases
Abstract Through extravasation of plasma and recruitment of leukocytes the airway microcirculation is critically involved in the development of an airway inflammation. Recent data indicate that vascular functions may be enhanced in inflamed airways as a result of angiogenesis and vascular remodelling. The present study tests the hypothesis that, similar to infectious airway conditions, the development of an allergic inflammation evokes prompt changes in vascular structure. Mice were sensitized to OVA day 0. At days 14-20 the mice were subjected to daily exposure of aerosolized allergen. Vessel diameters in tracheal whole-mount preparations were identified by iv administration of FITC-labelled Lectin, (L. esculentum).The experiment was terminated 24h after the last exposure and tissue samples were collected for assessment of lung eosinophilia, goblet cell metaplasia, and vascular changes. Allergic airway inflammation was recorded as lung tissue eosinophilia (p<0.01) and goblet cell metaplasia (p<0.01). The mean diameter of capillaries was increased in allergen challenged animals (11.3.±]0.2 um) compared to saline exposed controls (7.3.±]0.4 um, p<0.01). Also, numbers of cells in the lungs that expressed the proliferation marker PCNA increased (p<0.001). PCNA-stained nuclei occurred in airway epithelial cells, bronchial and vascular smooth muscle and the endothelium. In summary, this study indicates that development of an allergen-induced inflammation may be associated with prompt vascular remodeling involving increased mean capillary diameter and possibly an increased proliferative response of endothelial cells.
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K. Rydell, L. Uller, A. Bengtsson, C. G. A. Persson, J. S. Erjefält (Lund, Sweden). Development of allergic airway inflammation may be associated with prompt structural changes of small blood vessels. Eur Respir J 2002; 20: Suppl. 38, 293
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