Source: Annual Congress 2002 - Effects of air pollution on lung cells
Disease area: Airway diseases

Abstract

Ozone (O₃) is a common air pollutant associated with adverse health effects. Asthmatics have been suggested to be a particularly sensitive group. This study evaluated whether bronchial epithelial cytokine expression would differ between healthy and allergic asthmatics after ozone exposure, representing an explanatory model for differences in susceptibility.
Methods: 15 mild allergic asthmatic subjects using only inhaled β₂-agonists prn participated together with 14 healthy subjects in blinded and randomised exposures to 0.2 ppm ozone and filtered air for 2 hours. Bronchoscopy with bronchial mucosal biopsies was performed 6 hours after exposure. Biopsies were embedded in GMA and stained with mAbs for IL-5, IL-6, IL-8, IL-10, TNF-α, GRO-α, GM-CSF and ENA-78.
Results: When comparing the two groups at baseline, the asthmatic subjects showed an increased staining of IL-5 whereas the healthy demonstrated increases in IL-8, ENA-78, GRO-α, IL-10 and TNF-α. The stainings were generally reduced in the bronchial epithelium of healthy subjects at 6 hours after ozone exposure as compared to air but reached statistical significance for only TNF-α. In contrast, epithelial stainings for ENA-78, GM-CSF, IL-8 and IL-5 increased in asthmatics after ozone.
Conclusion: Mild asthmatics differed from healthy individuals in bronchial epithelial cytokine expression at 6 hours after ozone exposure. The cytokine production in the asthmatic epithelium was directed towards recruitment of eosinophils and neutrophils to the airways, which may result in a subsequent worsening of the asthmatic airway inflammation.

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J. Bosson, N. Stenfors, J. Pourazar, A. Blomberg, A. J. Frew, R. Helleday, F. J. Kelly, S. Wilson, T. Sandström (Umeå, Sweden; Southampton, London, United Kingdom). Ozone induced bronchial epithelial cytokine expression differs between asthmatic and healthy subjects. Eur Respir J 2002; 20: Suppl. 38, 266

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