Source: Annual Congress 2002 - Effects of air pollution on lung cells
Disease area: Airway diseases

Abstract

Vitamin supplementation has been reported to protect against ozone-induced symptoms. To investigate this we exposed subjects to ozone and air in a double-blinded, cross-over control study, with and without vitamins. Fourteen subjects were exposed on three occasions, to filtered-air [A], ozone (2-hours, 0.2-ppm) after placebo treatment [O+P] and ozone after 7-days supplementation with 500mg vitamin C (vitC), 100mg vitamin E (vitE)/day [O+V]. Nasal lavage was performed pre- and at 5.5-hours post-exposure, bronchoscopy with bronchial wash (BW) and bronchoalveolar lavage (BAL) 6-hours post-challenge. Lavage fluid was analysed for vitC and vitE to determine whether differences in RTLF antioxidant concentration or response could provide an explanation for their reported protective action. Supplementation failed to increase vitC concentrations in the pre-exposure nasal RTLF: 0.78(0.11-2.24)[A], 1.56 (0.26-2.59)[O+P], 1.43 (0.22-3.87)μM[O+V], {median+IQR}. However, a significant increase (P<0.01) in nasal RTLF vitC was observed in the supplemented group six-hours post-ozone: 1.10(0.41-4.58)[A], 1.80(0.37-3.67)[O+P], 2.57 (0.80-3.58)μM[O+V]. A similar adaptive vitC response was seen in BW after ozone, with an additional movement in the supplemented group: 0.63 (0.38-0.75)[A], 0.88 (0.53-1.11)[O+P], 1.10 (0.93-1.27)μM[O+V]. These data show that supplementation does not increase basal RTLF antioxidant concentrations, but rather provides an enhanced reserve. This can be mobilised to the surface of the lung under conditions of oxidative stress providing a mechanism to offset the harmful effects of ozone.

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I. S. Mudway, A. Behndig, A. Blomberg, R. Helleday, T. sandstrom, F. Kelly (London, United Kingdom; Umea, Sweden). Increased adaptive movements of antioxidants into the respiratory tract lining fluid (RTLF) of vitamin supplemented individuals following ozone challenge. Eur Respir J 2002; 20: Suppl. 38, 265

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