Source: Annual Congress 2002 - Lung cancer biology
Disease area: Thoracic oncology

Abstract

Recently we described amplification of the house keeping gene β-actin by PCR from exhaled breath condensate (EBC) from patients with non small cell lung cancer (NSCLC) and volunteers. Thus gene expression analysis in EBC is feasible. We now attempted to detect mutations of the tumor suppressor gene p53 in EBC of NSCLC patients.
EBC was collected from 13 patients with NSCLC. Human β-actin was detected by PCR. 3μl of native EBC were used for nested PCR (40 cycles) of exons five to eight of the p53 gene. p53 PCR product was sequenced using a 373A DNA Sequencer from Applied Biosystems.
β-actin was detected in 11 of 13 NSCLC patients (85%). Exon 5 of the p53 gene was amplified in 77%, exon six in 62%, exons seven and eight in 54% each. Mutations were found in four of the 13 patients (31%; three mutations in exon 6, one mutation in exon 8).
We here demonstrate the ability to detect mutations of p53 gene in EBC of patients with manifest NSCLC. Further investigations to evaluate expression analysis of tumor suppressor genes or oncogenes in high risk patients may be warranted for screening or follow up reasons.

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Citations should be made in the following way:
C. Gessner, H. Kuhn, K. Toepfer, S. Hammerschmidt, J. Schauer, H. Wirtz (Leipzig, Germany). Detection of p53 mutation in exhaled breath condensate from patients with nonsmall cell lung cancer. Eur Respir J 2002; 20: Suppl. 38, 222

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