Cell physiological role of HIF-1a and PTEN in adenocarcinoma cells in normoxia/hypoxia
F. Rose, B. Reichmann, S. Krick, F. Grimminger, R. Schermuly, L. Fink, N. Weissmann, W. Seeger, J. Haenze (Giessen, Germany)
Source: Annual Congress 2002 - Lung cancer biology
Session: Lung cancer biology
Session type: Oral Presentation
Number: 221
Disease area: Thoracic oncology
Abstract Aims: Our aim was to explore the role of the hypoxia-inducible-factor HIF-1a and the tumorsuppressorgene PTEN regarding hypoxia driven gene expression, cell proliferation and apoptosis in adenocarcinoma cells. Methods: For this purpose we selected H23 and A549 cells which were stable transfected by HIF-1a or PTEN using expression-plasmids These cells were then studied by measurement of 1) HIF-1 dependent gene expression using reporter gene assay, of 2) various proliferation tests (counting of cell number and colony formation, MTT test, anchorage independent growth by soft-agar-cloning, cell survival, BrdU-incorporation, and 3) apoptosis in hypoxia and during reoxygenation. Results and conclusions: The data show that HIF-1a overexpression suppresses cell growth in normoxia, and PTEN inhibits tumor formation in vitro (soft-agar-cloning). Furthermore HIF-1a inhibits apoptosis in hypoxia and activates cell cycle entry during reoxygenation, whereas PTEN favors tumor regression by strong inhibition of anchorage independent growth and decreasing cell survival in hypoxia.
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F. Rose, B. Reichmann, S. Krick, F. Grimminger, R. Schermuly, L. Fink, N. Weissmann, W. Seeger, J. Haenze (Giessen, Germany). Cell physiological role of HIF-1a and PTEN in adenocarcinoma cells in normoxia/hypoxia. Eur Respir J 2002; 20: Suppl. 38, 221
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