Source: Annual Congress 2002 - Lung cancer biology
Disease area: Thoracic oncology

Abstract

Cell surface receptors for laminin mediate cell adhesion to basal membranes, a critical step in the metastasis cascade. The 67 kDa high affinity laminin receptor (67LR), a non-integrin laminin receptor, is overexpressed in a large variety of human cancers and is associated with the invasive and metastatic phenotype. We studied the expression and the function of 67LR in cell lines, tissues and primary cell cultures derived from non-small cell lung carcinoma (NSCLC). The 67LR was highly expressed in lung mucoepidermoid- (NCI-H292), epidermoid- (Calu-1) and adeno- (GLC-82) carcinoma cell lines, while it was absent in the immortalized normal lung cell line MRC-5. The expression of 67LR was also increased in 21 out of 34 tumor tissues derived from patients affected by NSCLC (62%), as compared to adjacent normal tissues. In addition, primary cell cultures were prepared from normal and tumor tissues of 7 NSCLC patients: tumor cells showed increased expression of 67LR in 6 patients, as compared to the respective normal cells. Tumor cells overexpressing the 67LR showed an increased cell adhesion to laminin (LM), that could be dramatically inhibited by a recombinant polypeptide (r37LRP) containing laminin-binding domains of the 67LR. Therefore, the adhesion of NSCLC cells to LM was mostly mediated by the 67LR. Interestingly, the ability of NSCLC-derived cells to invade matrigel-coated membranes was also related to the level of 67LR expression and was specifically inhibited by r37LRP. These findings suggest that NSCLC overexpress a functional 67LR that conferes to the cells the ability to bind LM with high affinity and to migrate through basal membranes.

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A. A. Mancini, G. G. Marsico, A. A. Russo, P. P. Ragno, N. N. Montuori, G. G. Rossi, M. M. Caputi (Naples, Italy). Expression and function of the 67 kDa high-affinity laminin receptor in nonsmall cell lung carcinoma. Eur Respir J 2002; 20: Suppl. 38, 219

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