Source: Annual Congress 2013 –The pulmonary epithelium: recent developments
Disease area: Airway diseases

Abstract

Background: It is well known that essential oils of plants have stress palliation, diet and immunostimulation effects. However, little is known about the biological effects of essential oils on respiratory epithelial cells. Objectives: To evaluate the biological effects of essential oils on respiratory epithelial cells, we have examined cytotoxic, apoptotic and antioxidant activity in components of essential oils using a primary culture of alveolar type II cells in vitro. Our final goal is to examine applications of these potent essential oil components to respiratory health. Method: Alveolar type II cells were isolated from Fisher 344 male rats using elastase inserted into the trachea. The cells were exposed to components of essential oils with and without induced oxidative stress by the addition of H₂O₂ in the presence of Fe²⁺ ion for 6 hrs. At the end of the experiment, lactate dehydrogenase (LDH) activity released into the culture medium was determined to reflect the cytotoxic and antioxidant activities. Results: Cytotoxic activity for geraniol, linalool, mentol, cedrol, nerolidol and borneol were increased dose-dependently up to a concentration of 300 ppm. Eugenol and isoeugenol exerted potent free radical scavenging activities. Conclusions: The results indicate that components of essential oils produce various biological activities in alveolar type II cells. Eugenol and its compound can be useful protective agents for oxidative respiratory disorder in alveolar regions.

Rating:

Share or cite this content

Citations should be made in the following way:
Y. Takano, M. Takeda, Y. Hinode (Kitakatsuragi-gun, Japan). Biological activities of components of essential oils to alveolar epithelial cells. Eur Respir J 2013; 42: Suppl. 57, 3133

Member's Comments

No comment yet.

Related content which might interest you: Antioxodants attenuates alveolar epithelial cell damage induced by a component of essential oils Source: International Congress 2014 – Experimental models in acute lung injury Year: 2014 Mitochondrial function in three different types of airway epithelial cells Source: International Congress 2015 – Translational studies in airway cell biology Year: 2015 Cell intrinsic and environmental factors influencing human bronchial epithelial cell migration Source: International Congress 2016 – Lung cancer and immune cells: friends or foes? Year: 2016 Human lung mast cells alter asthmatic airway epithelial cell function Source: Annual Congress 2013 –The pulmonary epithelium: recent developments Year: 2013 Isolation and characterization of putative human lung alveolar epithelial progenitor cell from tissue explants Source: Annual Congress 2013 –The pulmonary epithelium: recent developments Year: 2013 Carbocysteine reverts the effects of cigarette smoke on the growth and on the senescence of bronchial epithelial cells Source: International Congress 2014 – Effect of cigarette smoke on cell behaviour: cell biology, signalling and senescence Year: 2014 Generation of an immortalized functional alveolar epithelial cell line originating from human induced pluripotent stem cells Source: International Congress 2016 – Common mechanisms in lung development and fibrosis Year: 2016 PINK1-Parkin pathway-mediated mitophagy is involved in cigarette smoke extract (CSE)-induced cellular senescence in human bronchial epithelial cells (HBEC) Source: International Congress 2014 – Effect of cigarette smoke on cell behaviour: cell biology, signalling and senescence Year: 2014 ABCA3 mutations disturb lipid homeostasis in alveolar epithelial cells Source: Annual Congress 2013 –The pulmonary epithelium: recent developments Year: 2013 Aspiration, epithelial injury and lung diseases Source: International Congress 2015 – New insights into lung disease pathogenesis Year: 2015 Effect of respiration on primary human alveolar epithelial cells Source: International Congress 2015 – Translational studies in airway cell biology Year: 2015 Parkin-mediated mitophagy is involved in cigarette smoke extract (CSE)-induced cellular senescence in human bronchial epithelial cells (HBEC) Source: Annual Congress 2013 –Stress responses and T-cell behaviour in the lung Year: 2013 Reconstituted human airway epithelium 3D-model to assess the impact of air pollutants on the inflammatory response Source: International Congress 2014 – Mechanistic studies of the airway epithelium Year: 2014 Involvement of mitochondrial fragmentation in cigarette smoke induced-bronchial epithelial cell senescence Source: Annual Congress 2013 –Stress responses and T-cell behaviour in the lung Year: 2013 Different stages of wound repair in differentiated human bronchial epithelial cells show altered microRNA expression Source: International Congress 2015 – Airway remodelling: recent developments Year: 2015 NF-κB-EGFR cross talk in lung cell proliferation Source: International Congress 2014 – Cell biology 2014 Year: 2014 Cigarette smoke affects differentiation and function of primary human bronchial epithelial cells Source: International Congress 2014 – Effect of cigarette smoke on cell behaviour: cell biology, signalling and senescence Year: 2014 Adaptation of lung epithelial cells subjected to chronic cyclic strain Source: International Congress 2014 – Mechanistic studies of the airway epithelium Year: 2014 The capacity of human airway epithelium to regulate T cell proliferation is reduced in asthma Source: International Congress 2015 – Asthma mechanisms and management Year: 2015 Involvement of PKCeta activation in cigarette smoke extract induced apoptosis in human lung fibroblasts (MRC-5 cell) Source: International Congress 2014 – Lung cell biology in injury and repair Year: 2014