Source: Annual Congress 2003 - Molecular biology of chronic lung disease and lung cancer
Disease area: Thoracic oncology

Abstract

Although risk factors for carcinomas of the lung are well recognized, very little is known about the molecular mechanisms responsible for this malignancy. cDNA microarrays are capable of profiling gene expression patterns of thousands of genes and/or gene clusters. However, this system is limited by the large amount of RNA required per hybridization. Therefore, we analyzed gene expression profiles of 20 sets of surgical specimen of adenocarcinomas of the lung and corresponding non-neoplastic lung tissue from the same individual using amplified mRNA to constitute molecular genetic portraits of those tumors. Gene expression profiles were examined using a 5000-element cDNA microarray.
Total RNA was extracted from frozen normal or cancer tissue. mRNA was amplified from total RNA and used for hybridisation. A panel of 20 primary adenocarcinomas were analysed with a reference RNA derived each from their corresponding non-neoplastic lung tissues. Initially, linear amplification of mRNA by used amplification procedure were controlled using total RNA and mRNA of two cell lines.
The data revealed 37 genes with high significantly different expression between cancer and non-neoplastic lung tissue. Using a threshold of 4-fold ( fold change=fc ≥ 4.0) over- or underexpression 23 genes remained. These include topoisomerase II alpha, genes involved in ubiquitination, LIM-domain proteins and other several transcription genes, showing diversity of gene expression in adenocarcinoma.
Funded by BMBF 1GC6901/5

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C. Seifart, U. Clostermann, O. Hartmann, T. Goldmann, E. Vollmer, D. Branscheid, C. Vogelmeier (Marburg, Borstel, Germany). Analysis of gene expression profiles of lung adenocarcinomas using amplified mRNA. Eur Respir J 2003; 22: Suppl. 45, 1506

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