Source: Annual Congress 2003 - Molecular biology of chronic lung disease and lung cancer
Disease area: Airway diseases

Abstract

Corticosteroids are potent anti-inflammatory agents widely used in asthma. It is known corticosteroids to activate eosinophil apoptotic death, but exact mechanisms of this effect are not established. Interleukin-12 (IL-12) activates production of apoptosis inducers interferon-γ and tumor necrosis factor-α. It was recently speculated IL-12 to play important role in cell death regulation. Present study was intended to evaluate the expression of mRNA of IL-12 receptor (IL-12R) (β1 and β2 subunits) and eosinophil apoptosis in induced sputum of 25 moderate asthmatics aged 7- 14 before and after fluticasone propionate 125 mcg twice daily for two weeks. Methods: RT-PCR was used to evaluate mRNA expression; sputum induction was performed using an inhalation of an aerosol of hypertonic saline at increasing concentrations (3, 4 and 5%); eosinophil apoptosis was assessed by morphologic evaluation of nuclear and cytoplasmic condensation. Results and conclusion: after two weeks of fluticasone treatment the activation of IL-12R mRNA expression (both β1 and β2 subunits) was found (P < 0.05) and this activation was accompanied by increase in eosinophil apoptotic death (P < 0.01). After the treatment IL-12R mRNA expression was highly correlated with number of apoptotic eosinophils (r = 0.71, P < 0.05 and r = 0.53, P < 0.05 for β1 and β2 subunits respectively). Thus we speculate IL-12 and/or its receptor expression can participate in corticosteroid depended activation of eosinophil apoptosis.

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F. I. Petrovski, I. I. Ivanchuk, A. E. Sazonov, I. A. Petrovskaia, L. M. Ogorodova (Tomsk, Russia). Interleukin-12 receptor mRNA expression and corticosteroid depended eosinophil apoptosis in asthma. Eur Respir J 2003; 22: Suppl. 45, 1497

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