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Vienna 2003
Monday 29.09.2003
Molecular biology of chronic lung disease and lung cancer
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Expression of ADAM33 splice variants in bronchial biopsies from normal and asthmatic subjects
H. M. Haitchi, R. M. Powell, T. J. Shaw, S. K. Babu, P. H. Howarth, S. T. Holgate, D. E. Davies (Southampton, United Kingdom)
Source:
Annual Congress 2003 - Molecular biology of chronic lung disease and lung cancer
Session:
Molecular biology of chronic lung disease and lung cancer
Session type:
Poster Discussion
Number:
1492
Disease area:
Airway diseases
Abstract
Rationale:
ADAM33
is the newest member of the A Disintegrin and Metalloprotease (ADAM) family and polymorphic variation in
ADAM33
has recently been shown to be strongly associated with asthma and bronchial hyperresponsiveness (VanEerdewegh et al, Nature 2002;418(6896):426-30). As ADAM33 is expressed in airway mesenchymal cells and exists in several alternatively spliced forms, we postulated that multiple splice variants of ADAM33 are expressed in bronchial tissue.
Methods:
Bronchial biopsies were obtained from normal (n=4) and asthmatic (n=5) adult volunteers after fully informed consent and local ethical approval. RNA was extracted from the biopsies and expression of ADAM33 splice variants quantified by real time PCR using specific primer-sets.
Results:
ADAM33 expression was detected in all bronchial biopsies with a trend towards greater expression of all splice variants in asthmatic compared to control subjects. The beta form which has been identified in mouse and human isolates was an extremely rare transcript, as were transcripts encoding exons within the metalloprotease domain (MP).
Conclusion:
All ADAM33 splice variants identified to date were detectable in bronchial biopsies. The low abundance of transcripts encoding the MP domain in bronchial biopsies is consistent with previous findings in cultured mesenchymal cells, suggesting key roles for other domains in this multifunctional protein.
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H. M. Haitchi, R. M. Powell, T. J. Shaw, S. K. Babu, P. H. Howarth, S. T. Holgate, D. E. Davies (Southampton, United Kingdom). Expression of ADAM33 splice variants in bronchial biopsies from normal and asthmatic subjects. Eur Respir J 2003; 22: Suppl. 45, 1492
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