Source: Annual Congress 2003 - Molecular biology of chronic lung disease and lung cancer
Disease area: Airway diseases

Abstract

The Transforming Growth Factor (TGF)-β superfamily regulates essential cellular processes, including proliferation and differentiation. Information about specific expression profiles of this system in the lung, however, is not available yet. Here, we generated a lung compartment-specific expression analysis of the TGF-β/BMP system using a combination of laser microdissection, cDNA array analysis, and RT-PCR. We analyzed RNA expression patterns in smooth muscle, airway epithelium, and alveolar septae in vivo. Out of 112 genes specific for TGF-β/BMP pathway, the following were highly expressed in all four compartments: JunB, InhibinA, Id2, Gdf9, Sox4, Gdf3, Bmp3, Bmp4, Bmp6, Col3a1, Bmp8a, Bmp8b. A number of genes, however, were selectively expressed in distinct compartments. ActRII and ALK-1 were notably absent in airway epithelium, Smad1 was absent in smooth muscle, and TGF-β3 was absent in alveolar septa, when compared to the other compartments. These findings were further confirmed by immunohistochemistry. Using RT-PCR with RNA generated from microdissected fresh-frozen tissue, we were furthermore able to selectively detect and clone full-length coding regions of TGF-β and BMP receptors. Interestingly, all tissues exhibited a single isoform of ALK-1, TβRII, and BMPRII. This study is thus a further step towards our understanding of the TGF-β/BMP system in the lung. It further demonstrates the feasibility of cloning full-length cDNAs from microdissected tissue compartments of the lung, enabling us to identify single RNA molecules from diseased lung compartments and comparing them to normal areas.

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O. V. Amarie, O. Eickelberg, L. Fink, W. Seeger, P. D. Shalamanov, M. E. Yeager (Giessen, Germany). Analysis of the TGF-β superfamily in mouse lung. Eur Respir J 2003; 22: Suppl. 45, 1490

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