Evaluation of cell spectrum in induced sputum of young cigarette smokers
A. Kislina, G. Strazda, L. Balode, Z. Sinkevica, D. Isajeva, S. Isajevs, N. Jurka, I. Taivans, V. Gordjusina, V. Silins (Riga, Latvia)
Source: Annual Congress 2012 - Tobacco and shisha exposure in children and adolescents
Session: Tobacco and shisha exposure in children and adolescents
Session type: Thematic Poster Session
Number: 4064
Disease area: Airway diseases
Abstract It is of great importance to be able to diagnose early inflammatory changes that can lead to COPD in airways of young cigarette smokers.Aim : The aim of this study was to analyze the cell spectrum of induced sputum in young cigarette smokers, with emphasis on T-regulatory cells.Method : 20 healthy non-allergic smokers (Mean Age - 22±0.84; regular usage - 2.60±1.18 pack-years), and 20 non-smokers gave informed consent to participate in the study. Lung function measurements, sputum induction (IS) and sputum cell analyses were preformed.Results : Demographic data for both study groups did not differ significantly. Both non-smokers and smokers had normal lung function indices. Induced sputum of smokers contained higher (p = 0.021) absolute amount of neutrophils [29.70 (20.96 – 42.09), cellsx104 /mL], compared with non-smokers [16.51 (11.65 – 23.39) cellsx104 / mL]. FOXP3-positive T reg cells in sputum of young smokers showed a trend towards statistically significant increase [11.10 (7.75 – 15.88) cellsx104 / mL], compared with non-smokers [6.79 (4.75 – 9.72) cellsx104 / mL, p = 0.057]. Regression analysis showed significant dependency of FOXP3-positive T reg cells (r2 = 0.103; p = 0.044), neutrophils (r2 = 0.179; p = 0.007) and macrophages of induced sputum (r2 = 0.118; p = 0.030) from the number of smoked pack–years.Conclusion : This study demonstrates that young smokers have early inflammatory changes in their airways, which not only initiate non-specific mechanisms recruiting neutrophils, but also involve specific immune mechanisms with recruitment of T reg lymphocytes. The lymphocyte response is probably adaptive.
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A. Kislina, G. Strazda, L. Balode, Z. Sinkevica, D. Isajeva, S. Isajevs, N. Jurka, I. Taivans, V. Gordjusina, V. Silins (Riga, Latvia). Evaluation of cell spectrum in induced sputum of young cigarette smokers. Eur Respir J 2012; 40: Suppl. 56, 4064
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