Source: Annual Congress 2004 - Lung function technology: beyond the basic test
Disease area: Airway diseases

Abstract

Aims: (1) To compare the concentration of nitrite in exhaled breath condensate (EBC), BALF and blood.
(2) To evaluate the influence of food intake.
Methods: In 12 pigs used as a model, 48 EBC and 48 serum samples were collected 2-4 hours after feeding. At the end of the study, EBC, blood and BALF were collected in non-fed animals (n = 12). For EBC collection, the "ECoScreen" (Viasys) was used. Ventilatory variables (expiratory flow, respiratory rate, tidal volume) were registered in parallel. Nitrite was analysed using the Griess method. The calibration curve was linear between 0.5 – 15 µmol/l.
Results: No influence of food intake was determined on the concentration of nitrite in either serum or EBC. In the peripheral blood, normally distributed nitrite concentrations were observed ranging from <0.5 to 6.0 μmol/l (average ± standard deviation: 2.46 ± 1.26 µmol/l). In EBC, nitrite concentration differed significantly between individuals but was highly reproducible within each animal. The inter-individual range was 0.3 – 6.8 µmol/l while the intra-individual variability was found to be much lower. Nitrite concentration in EBC was not significantly influenced by variables of ventilation during the EBC sampling procedure. In BALF, the concentration of nitrite was ≤ 2.0 µmol/l (average ± standard deviation: 1.34 ± 0.48 µmol/l, n=10) with two outlier values (6.6 and 7.1µmol/l) in morphologically normal lungs. No significant correlation was seen between the nitrite concentration in EBC and those in either BALF or blood serum, nor between BALF and blood serum.

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Citations should be made in the following way:
P. Reinhold, A. Langenberg, G. Foedisch, M. Rothe (Jena, Berlin, Germany). The physiological concentration of nitrite in exhaled breath condensate, broncho-alveolar lavage fluid and blood samples. Eur Respir J 2004; 24: Suppl. 48, 2485

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