Source: Annual Congress 2011 - Bronchoalveolar lavage and biomarkers in diffuse parenchymal lung disease
Disease area: Interstitial lung diseases

Abstract

We have previously reported increased frequency of haemosiderin-laden alveolar macrophages in patients whit IPF in comparison to other infiltrative lung diseases.
We performed a retrospective analysis of 49 patients who received diagnosis of IPF. The diagnosis of IPF were in accord to the ATS/ERS guidelines. BAL was performed in all subjects.We evaluated the occurrence of haemosiderin-laden alveolar macrophages in patients whit IPF.Perls Prussian blue stain was used to detect haemosiderin laden alveolar macrophages. ≥400 M Ø were examined for the number of cells that stained with Perls‘ Prussian Blue stain, and a percentage score was estabilished by dividing the number of Prussian-blue-positive cells by the total number of cells counted. Perls positivity were defined as percentage of BALF M Ø > 50%. Patients were divided in 2 groups based on BALF Perls positivity: IPF BALF MØ P+ 15/49 (30%) and IPF BALF MØ P – 24/49 (70%).Variables compared between the 2 groups included cellular BALF data, pulmonary function tests, arterial blood gases, and PAPs estimated by echocardiography.
DLCO% th and% oxygen desaturation during 6MWT were significantly reduced in IPF BALF MØ P+ in comparison to IPF BALF MØ P- (respectively 29 ± 9 vs 41 ± 14, p=0,03; and 18 ± 5 vs 12 ±1, p= 0,02) while FVC%th (68 ±18 vs 71 ±19) and resting paO2 mmHg 64 ± 17 vs 69 ± 9) were not significantly different.
Conclusions: Increased haemosiderin laden alveolar macrophages is associated to more severe impairment of lung diffusion and higher oxygen desaturation during exercise.

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Citations should be made in the following way:
C. Vitale, E. Carpentieri, F. Perna, M. Pitassi, A. Molino, A. Stanziola, M. Sofia (Naples, Italy). Clinical and functional features in idiopathic pulmonary fibrosis (IPF) with and without haemosiderin-laden alveolar macrophages on BALF. Eur Respir J 2011; 38: Suppl. 55, 4769

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