Detection of pseudomonas aeruginosa (Pa ) specific peaks by ion mobility spectrometry (IMS) in exhaled breath of bronchiectasis patients
T. Rabis, O. Anhenn, J. I. Baumbach, I. Kurth, G. Weinreich, H. Teschler, L. Freitag, K. Darwiche, U. Mellies, P. M. Rath, U. Sommerwerck (Essen, Saarbrücken, Germany)
Source: Annual Congress 2011 - Role of the biomarkers in airway diseases
Session: Role of the biomarkers in airway diseases
Session type: Poster Discussion
Number: 4790
Disease area: Airway diseases, Respiratory infections
Abstract Introduction Colonisation or infection of airways from bronchiectasis patients by Pa results in chronic inflammation leading to a progressive destruction of the lung and to a decline in lung function. Therefore more inpatient stays for intravenous antibiotic treatment are necessary and the quality of life in these patients is severely limited.Objectives Aim of our study was to detect and compare volatile organic compounds (VOCs) by IMS in exhaled breath of bronchiectasis patients either colonised or infected by Pa with healthy non-smoking controls (hc).Methods We have analysed VOCs by IMS coupled to a multi-capillary column (MCC) for pre-separation (MCC-IMS, B&S Analytik) in exhaled breath of bronchiectasis patients either colonised (Pac,n=3) or infected (Pai,n=9) by Pa (Pac+Pai=Pa+,n=12) compared to hc (n=39) and compared Pac with Pai. In addition we analysed VOCs from Pa cultures growing on agar plates.Results Using IMS for VOC analysis, differences between Pa+ and hc could be found. Different peaks were detected between Pac and hc, Pai and hc as well as Pac and Pai. VOC analysis from Pa cultures revealed two peaks which could be found in the Pa+ bronchiectasis patients.Conclusions IMS seems to be a promising method for the non-invasive identification of patients which are colonized or infected with Pa . A differentiation between patients colonised or infected with Pa seems to be possible, as well. However, confirmation of our findings in a larger study population is needed. The comparison of Pa+ with Pa cultures will help to identify peaks caused by the presence of Pa . Furthermore, it is required to identify the molecules representing the peaks.
Rating:
You must login to grade this presentation.
Share or cite this content
Citations should be made in the following way:
T. Rabis, O. Anhenn, J. I. Baumbach, I. Kurth, G. Weinreich, H. Teschler, L. Freitag, K. Darwiche, U. Mellies, P. M. Rath, U. Sommerwerck (Essen, Saarbrücken, Germany). Detection of pseudomonas aeruginosa (Pa ) specific peaks by ion mobility spectrometry (IMS) in exhaled breath of bronchiectasis patients. Eur Respir J 2011; 38: Suppl. 55, 4790
You must login to share this Presentation/Article on Twitter, Facebook, LinkedIn or by email.
Member's Comments
Related content which might interest you:
Related content which might interest you: