Metabolomic analysis of exhaled breath condensate in diagnostics of obstructive airway diseases
T. N. Anokhina, E. K. Anaev, A. A. Rodionov, A. I. Revelsky, I. A. Revelsky, V. B. Kudriavtsev, A. G. Chuchalin (Moscow, Russian Federation)
Source: Annual Congress 2011 - Role of the biomarkers in airway diseases
Session: Role of the biomarkers in airway diseases
Session type: Poster Discussion
Number: 4791
Disease area: Airway diseases
Abstract Metabolomic analysis provides molecular and biochemical profiles of metabolites in different biological fluids.Objectives: The aim of this study was to assess the potential of exhaled breath condensate (EBC) molecular profiling in discrimination patients with COPD and asthma and healthy subjects.Methods: Twenty patients with asthma, twenty patients with COPD and thirty healthy control subjects were enrolled in cross-sectional study. Every subject performed spirometry and EBC collection. EBC samples were analyzed by gas chromatography – mass-spectrometry method (GC-MS). EBC profiles from patients with asthma were separated from patients with COPD and from healthy control subjects using an algorithm based on linear methods of pattern recognition theory.Results: We have detected various profiles of semi-volatile organic compounds (SvOC) in EBC in patients with asthma, COPD and healthy subjects. Mathematical approach to available data revealed 9 SvOC which have been deemed the most appropriate for solving recognition problem (2-phenoxyethanol, decanol-1, ethyl citrate, 2,3–dihydro-1-H-inden-1-on and others). EBC profiles of healthy subjects can be distinguished from patients with asthma with reliability 75%, healthy subjects from COPD patients with reliability 85% and asthma patients from COPD patients with reliability 83%.Conclusion: Metabolomic analysis of EBC can discriminate patients with asthma and COPD and healthy subjects. We propose that differences in SvOC profiles between asthma and COPD are disease related.
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T. N. Anokhina, E. K. Anaev, A. A. Rodionov, A. I. Revelsky, I. A. Revelsky, V. B. Kudriavtsev, A. G. Chuchalin (Moscow, Russian Federation). Metabolomic analysis of exhaled breath condensate in diagnostics of obstructive airway diseases. Eur Respir J 2011; 38: Suppl. 55, 4791
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