Source: Annual Congress 2011 - Bronchoalveolar lavage and biomarkers in diffuse parenchymal lung disease
Disease area: Interstitial lung diseases

Abstract

It is long recognised that lung cancer has an increased frequency in idiopathic pulmonary fibrosis (IPF), raising questions regarding the similarity of IPF and lung cancer biology. Matrix metalloproteinases regulate remodelling of the extracellular matrix, an important function for pathological processes such as angiogenesis, tissue repair and tumor invasion. We aim to assess the expression of this pathway in BALF samples of lung cancer patients and compare with IPF patients to examine possible pathogenetic links between these two lethal pulmonary diseases.
We prospectively studied 23 newly diagnosed patients, with non small cell lung cancer (NSCLC), 10 IPF patients and 10 healthy controls (C). MMP2, MMP7, MMP9, TIMP1 and TIMP2 mRNA expression levels were measured in BALF by real time RT-PCR. MMP7 protein levels were measured in BALF supernatants using ELISA kit.
mRNA expression of MMPs 2,7,9 and TIMP1 was significantly increased in lung cancer compared to controls (p<0.05). The IPF population showed decreased expression of the aforementioned MMPs in comparison with lung cancer while did not show significant difference at the mRNA level in comparison to controls. MMP7 protein levels (pg/μl) were significantly higher in both NSCLC and IPF populations compared to controls (NSCLC: 24.69±4.18, IPF: 18.65±2.11, C=9.76±1.92, with p values: NSCLCvsC p=0.032, IPFvsC p=0.005).
Increased expression of MMP-7 in both m-RNA and protein level may suggest a common link between those lethal disorders. Given that our population was newly diagnosed, the over-expression of these markers could be predictive of tumor progression, migratory behaviour and metastasis potential.

Rating:

Share or cite this content

Citations should be made in the following way:
K. Samara, I. Giannarakis, I. Papanikoalou, I. Lambiri, N. Siafakas, K. Antoniou (Heraklion, Greece). Overexpression of matrix metalloproteinase-7 (MMP-7) in bronchoalveolar lavage fluid (BALF) of IPF and lung cancer patients. Eur Respir J 2011; 38: Suppl. 55, 4761

Member's Comments

No comment yet.

Related content which might interest you: Matrix metalloproteinase-9 (MMP-9) elevated in serum, but not in bronchial lavage in lung cancer patients Source: Eur Respir J 2005; 26: Suppl. 49, 328s Year: 2005 Matrix metalloproteinase-9 (MMP-9) elevated in serum, but not in bronchial lavage in lung cancer patients Source: Annual Congress 2013 –New methods for diagnostic workup of lung cancer Year: 2013 Elevated level of tissue inhibitor of metalloproteinase 1 in bronchoalveolar lavage fluid in fibrosing lung diseases Source: Eur Respir J 2002; 20: Suppl. 38, 313s Year: 2002 TNF-alpha, IL-1 and IL-6 concentration in bronchoalveolar lavage fluid (BALF) of non-small cell lung cancer (NSCLC) Source: Annual Congress 2013 –New methods for diagnostic workup of lung cancer Year: 2013 Osteopontin and matrix metalloproteinase-7 in bronchoalveolar lavage as markers of idiopathic pulmonary fibrosis Source: Annual Congress 2010 - Usefulness of bronchoalveolar lavage in diffuse parenchymal lung disease Year: 2010 Induced sputum (IS) in patients with interstitial lung disease (ILD) – comparison with bronchoalveolar lavage (BAL) Source: Annual Congress 2007 - Clinical aspects of interstitial lung disease Year: 2007 Expression of matrix metalloproteinases in bronchoalveolar cells in COPD and in COPD phenotypes Source: International Congress 2015 – Monitoring and phenotyping COPD Year: 2015 CCR5 expression and CC-chemokine levels in broncho-alveolar lavage (BAL) fluid of patients with idiopathic pulmonary fibrosis (IPF) Source: Eur Respir J 2002; 20: Suppl. 38, 598s Year: 2002 Elevated TGF- b1 concentration in bronchoalveolar lavage fluid from patients with primary lung cancer Source: Eur Respir J 2002; 20: Suppl. 38, 585s Year: 2002 Measurement of trefoil factor peptides in bronchioalveolar lavage (BAL) fluids from patients with COPD or pulmonary cancer Source: Annual Congress 2009 - COPD: basic science and clinical studies Year: 2009 Evaluation of IL-6 level in serum and bronchoalveolar lavage fluid of patients with non-small cell lung cancer (NSCLC) Source: Annual Congress 2011 - Biomarkers and other new methods for lung cancer Year: 2011 Fibrocytes are detected in bronchoalveolar lavage (BAL) fluid in idiopathic pulmonary fibrosis (IPF) Source: Annual Congress 2011 - Bronchoalveolar lavage and biomarkers in diffuse parenchymal lung disease Year: 2011 Determination of a typical protein expression profile in bronchoalveolar lavage fluid (BALF) from patients with pulmonary sarcoidosis using SELDI-mass spectrometry Source: Annual Congress 2005 - Progresses in granolomatous lung disease Year: 2005 Elevated transforming growth factor (TGF)-β1 and thrombospondin (TSP)-1 levels in bronchoalveolar lavage (BAL) fluid of patients with pulmonary fibrosis Source: Eur Respir J 2003; 22: Suppl. 45, 83s Year: 2003 Studies on hepatocyte growth factor (HGF) in bronchoalveolar lavage (BAL) do not support the concept on its antifibrotic properties in idiopathic pulmonary fibrosis (IPF) Source: International Congress 2015 – Interstitial lung diseases II Year: 2015 Net inflammatory activity of bronchoalveolar lavage fluid (BALF) from patients following lung transplantation Source: Eur Respir J 2002; 20: Suppl. 38, 348s Year: 2002 Induced sputum versus bronchoalveolar lavage fluid (BALF) in patients with idiopathic pulmonary fibrosis (IPF) Source: Eur Respir J 2003; 22: Suppl. 45, 227s Year: 2003 Prognostic value of cytokeratin 19 in bronchoalveolar lavage (BAL) in patients with acute lung injury (ALI) Source: Eur Respir J 2002; 20: Suppl. 38, 599s Year: 2002 Lymphocyte and macrophage apoptosis in bronchoalveolar lavage fluid (BALF) of COPD patients Source: Eur Respir J 2006; 28: Suppl. 50, 749s Year: 2006 Evaluation of IL-10 level in serum and bronchoalveolar lavage fluid of patients with non-small cell lung cancer (NSCLC) Source: Annual Congress 2013 –Prognostic factors and subtyping of lung cancer Year: 2013