Source: Annual Congress 2011 - Bronchoalveolar lavage and biomarkers in diffuse parenchymal lung disease
Disease area: Interstitial lung diseases

Abstract

Introduction and Aims:
Idiopathic Pulmonary Fibrosis (IPF) and Hypersensitivity Pneumonitis (HP) are both interstitial lung diseases characterized by a mixture of inflammation and fibrosis. We aim to investigate the role of different pathophysiological mechanisms by analysing broncho-alveolar lavage (BAL) fluid samples.
Methods:
Bronchoscopy with BAL (4 aliquots of 50 ml saline, fraction 2-4 pooled for analysis) performed at time of diagnosis were evaluated and 3 groups; HP (n=11), IPF (n=11) and control (n=10) were analyzed by multiplex ELISA (SearchLight®). Kruskal-Wallis one-way ANOVA with Dunns post-hoc test was used for analysis.
Results:
Results are displayed in the table. Differences were found regarding total lymphocyte, neutrophil and eosinophil cell counts. Differences were found in pathological mechanisms of coagulation (Protein-C, PAI-1 Active), angiogenesis (VEGF), inflammation (MCP-1, MDC, IL-8, IL-12p40), fibrosis (TGF-β1, HGF), matrix remodeling pathways (MMP-8, MMP-9) and oxidative stress (MPO).
Conclusion:
Differences for aforementioned mechanisms were observed in IPF and HP compared to control, as well as discriminating factors between IPF and HP. These results highlight the role of BAL in the search for new biomarkers and therapies for pulmonary fibrosis.

Rating:

Share or cite this content

Citations should be made in the following way:
S. Willems, J. Somers, S. E. Verleden, G. M. Verleden, J. Yserbyt, C. Dooms, B. Vanaudenaerde, W. Wuyts (Leuven, Belgium). Proteological assesment of idiopathic pulmonary fibrosis and hypersensitivity pneumonitis by mean of broncho-alveolar lavage. Eur Respir J 2011; 38: Suppl. 55, 4767

Member's Comments

No comment yet.

Related content which might interest you: The value of lymphocytosis in bronchoalveolar lavage in the differential diagnosis between idiopathic pulmonary fibrosis and chronic hypersensitivity pneumonitis Source: Annual Congress 2011 - Work, the environment and respiratory disease Year: 2011 Bronchoalveolar lavage Source: Eur Respir Mon; 2010: 48: 59–72 Year: 2010 Calgranuline B in bronchoalveolar lavage of patients with idiopathic pulmonary fibrosis Source: Annual Congress 2009 - What is new in molecular pathology and functional genomics of neoplastic and non-neoplastic lung disease? Year: 2009 Bronchoalveolar lavage in idiopathic pulmonary fibrosis Source: International Congress 2017 – IPF: from the bench to the bedside Year: 2017 BAL findings in idiopathic nonspecific interstitial pneumonia and usual interstitial pneumonia Source: Eur Respir J 2003; 22: 239-244 Year: 2003 The decrease of surfactant protein D in bronchoalveolar lavage fluid in patients with idiopathic pulmonary fibrosis and nonspecific interstitial pneumonia Source: Annual Congress 2012 - Diffuse parenchymal lung disease III Year: 2012 The coagulation and antifibrynolitic activities are increased in the bronchoalveolar lavage fluid from patients with progressing idiopathic pulmonary fibrosis Source: Eur Respir J 2007; 30: Suppl. 51, 575s Year: 2007 Relationship between Ca19.9 levels in bronchoalveolar lavage, neutrophilic alveolitis, pulmonary function tests and HRCT pattern in pulmonary fibrosis Source: Eur Respir J 2003; 22: Suppl. 45, 588s Year: 2003 Bronchoalveolar lavage pepsin in acute exacerbation of idiopathic pulmonary fibrosis Source: Eur Respir J 2012; 39: 352-358 Year: 2012 Cytofluorimetric BAL cells characterization in IPF and in other ILD Source: International Congress 2016 – ILD: from the bench to the bedside Year: 2016 Impact of lymphocyte differential count in BALF on the mortality of patients with acute exacerbation of idiopathic chronic fibrosing interstitial pneumonia Source: International Congress 2016 – Clinical aspects of ILD Year: 2016 Clinical significance of hemosiderin-laden macrophages in bronchoalveolar lavage fluid of patients with idiopathic interstitial pneumonias Source: Annual Congress 2013 –Diffuse pulmonary fibrosis Year: 2013 Biomarkers of fibroproliferative healing in fibrosing idiopathic interstitial pneumonias Source: Annual Congress 2013 –Idiopathic pulmonary fibrosis: clinical view Year: 2013 Cytokine profiles of bronchoalveolar lavage fluid in patients with combined pulmonary fibrosis and emphysema Source: Annual Congress 2012 - Idiopathic pulmonary fibrosis Year: 2012 Effect of low level laser therapy in a model of pulmonary idiopathic fibrosis Source: International Congress 2015 – Pulmonary fibrosis: mechanisms of disease Year: 2015 Induced sputum versus bronchoalveolar lavage fluid (BALF) in patients with idiopathic pulmonary fibrosis (IPF) Source: Eur Respir J 2003; 22: Suppl. 45, 227s Year: 2003 Bronchoalveolar lavage findings and disease progression in hypersensitivity pneumonitis Source: International Congress 2017 – ILDs: clinical aspects Year: 2017 Secondary pulmonary alveolar proteinosis associated with IgG4-related disease Source: Annual Congress 2013 –Diffuse parenchymal lung disease II Year: 2013 Investigations of broncho-alveolar lavage in children with asthma, bronchiectasis and cystic fibrosis Source: International Congress 2015 – Paediatric bronchology Year: 2015