Optimization of a whole blood assay for determining cytokine responses in occupational populations

L. A. M. Smit, I. M. Wouters, D. Heederik, G. T. Rijkers, G. Doekes (Utrecht, Netherlands)

Source: Annual Congress 2005 - Organic dusts - warfare and disasters
Session: Organic dusts - warfare and disasters
Session type: Thematic Poster Session
Number: 3826
Disease area: Airway diseases

Congress or journal article abstract

Rating: 0
You must login to grade this presentation.

Share or cite this content

Citations should be made in the following way:
L. A. M. Smit, I. M. Wouters, D. Heederik, G. T. Rijkers, G. Doekes (Utrecht, Netherlands). Optimization of a whole blood assay for determining cytokine responses in occupational populations. Eur Respir J 2005; 26: Suppl. 49, 3826

You must login to share this Presentation/Article on Twitter, Facebook, LinkedIn or by email.

Member's Comments

No comment yet.
You must Login to comment this presentation.


Related content which might interest you:
Ex vivo cytokine release reflects sensitivity to occupational endotoxin exposure
Source: Eur Respir J 2009; 34: 795-802
Year: 2009



Development of a short-term whole blood intracellular staining assay to characterize the immune profile of pediatric patients
Source: International Congress 2017 – Pulmonary infection and inflammation
Year: 2017


High endotoxin activity in seed extracts associated with the capacity to induce in vitro cytokine production in a whole blood assay
Source: Eur Respir J 2002; 20: Suppl. 38, 392s
Year: 2002

Simple improvement of cell culture conditions significantly augments IP-10 and INF-γ responsiveness in a whole blood based CMI assay
Source: Annual Congress 2010 - Tuberculosis: from bench to bedside
Year: 2010

Cytokine production induced by soluble and particulate stimuli in human peripheral blood mononuclear cells: modulation by pharmacological agents
Source: Eur Respir J 2004; 24: Suppl. 48, 579s
Year: 2004

Alteration of the inflammatory response in blood ex vivo in pig farmers
Source: Eur Respir J 2006; 28: Suppl. 50, 567s
Year: 2006

Performance outcomes of standardized antigen-driven IFN-g release assays for the diagnosis of active TB in a high burden setting
Source: Annual Congress 2008 - Interferon-gamma release assay tests for diagnosing tuberculosis infection and disease
Year: 2008

Lymphocyte activation after exposure to swine dust: a role of humoral mediators and phagocytic cells
Source: Eur Respir J 2002; 19: 104-107
Year: 2002



Selection of blood samples in studies of respiratory diseases related to macrophage migration inhibitory factor
Source: Virtual Congress 2020 – Interesting clinical problems for the respiratory physician
Year: 2020

Intracellular cytokines in blood T cells and monocytes in lung transplant patients - potential utility in monitoring immunosuppression protocols
Source: Eur Respir J 2004; 24: Suppl. 48, 452s
Year: 2004

Low versus high Th2 asthma: variability of blood eosinophil counts in a longitudinal analysis
Source: Virtual Congress 2021 – Severe asthma: evaluation using patient-reported outcome measures (PROMs) and biomarkers, comorbidities and treatments
Year: 2021


Use of immune cell function assay in immune surveillance of lung transplant recipients: Correlations with clinical outcome and other immune assays
Source: Annual Congress 2012 - Optimising outcomes after lung transplantation
Year: 2012


Evaluation of a GR-ELISA for quantitative determination of glucocorticoid receptor activity in adherent cells and blood leukocytes
Source: Annual Congress 2009 - Models of chronic airways disease and therapy
Year: 2009

The relationship between pulmonary function tests and systemic cytokine response in animal food processing workers
Source: Eur Respir J 2002; 20: Suppl. 38, 393s
Year: 2002

Impact of blood eosinophil variability in asthma: a real-life population study.
Source: Virtual Congress 2021 – Clinical features and new biomarkers of asthma, COPD and chronic cough
Year: 2021


The role of the ESAT-6/CFP-10 in whole blood IFN-gamma test in TB assessment
Source: Eur Respir J 2006; 28: Suppl. 50, 140s
Year: 2006

Associations between lipid mediators and cytokine production at different stages of MTB infection
Source: Virtual Congress 2021 – Immunological and molecular characterisation of tuberculosis disease and infection
Year: 2021


The influence of family size and birth order on intracellular interferon (IFN)-γ production by cord blood mononuclear cells (CBMC): ²hygiene hypothesis² vs. ²intrauterine programming hypothesis² of atopy forming
Source: Annual Congress 2008 - Risk factors for childhood asthma and allergy
Year: 2008