Source: Annual Congress 2010 - State of the art imaging

Abstract

Optical Frequency Domain Imaging (OFDI) is a promising new modality for studying alveolar structure and function in vivo due to its high resolution (~10μm) and fast imaging speed (>100fps). Increasing the imaging depth of this technology is particularly important for investigating the static and dynamic interdependence of multiple connected alveoli.
We assessed the influence of numerical aperture (NA) and multi depth focusing on the maximum imaging depth in air filled alveoli. We hypothesized we could identify an optimal set of focusing parameters depending on the research question.
Four different NAs ranging from 0.02 to 0.1 were evaluated for three-dimensional imaging of alveolar networks. Furthermore, the influence of different focal depths was studied. Two rabbit lungs were instillation fixed and dried using a modified Heitzman fixation technique at 20 cm H₂O pressure. Three-dimensional data sets with a field of view of 1.33mm x 1.33mm were obtained using OFDI in the air filled upper right lobe. Images were analyzed to determine the best compromise between imaging depth, resolution and depth of field for the visualization of alveolar structures.
The imaging depths at which airway walls were clearly identified varied from 80 to 200 μm. Acquisition of multiple depths of focus resulted in an improved imaging depth for the 0.1 NA. The best overall compromise in a single data set was achieved with the 0.08 NA.
We have demonstrated that optimization of optical focusing parameters is crucial for imaging of alveolar networks. Further research to translate this knowledge to the in vivo setting is merited.

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Citations should be made in the following way:
C. Unglert, E. Namati, B. Bouma, G. Tearney (Boston, United States Of America; Les-Loges-en-Josas, France). Late Breaking Abstract: Variation of the focusing parameters for three-dimensional optical frequency domain imaging of the peripheral lung. Eur Respir J 2010; 36: Suppl. 54, 5275

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