Source: Annual Congress 2010 - Influenza A (H1N1) and other viral infections: therapeutic aspects
Disease area: Respiratory infections

Abstract

Influenza A virus (IAV) infection provokes the development of such host diseases as bacterial pneumonia, otitis, meningitis and the other severe complications. It has been known that IAV infection of human blood monocytes and neutrophils leaded to the decrease of phagocytosis; though, the definite mechanisms of IAV-induced immunity suppression have not been revealed yet. Mechanisms of ROS generation during the initial stage of IAV (FPV H7N1) interaction with neutrophils and monocytes have been studied in the present work.
Redox systems of IAV, ROS production by phagocytes and cell myeloperoxidase (MPO) activity were investigated using luminol-dependent chemiluminescent method. Cells were activated by adhesion to glass. Secretory degranulation of neutrophils was studied by lysozyme release into extracellular space using spectrophotometric method.
It was established that IAV had peroxidase-like activity, since IAV induced ROS generation in the system containing hydrogen peroxide and luminol. It was shown that IAV inoculation to stimulated to phagocytosis neutrophils and monocytes leaded to significant ROS formation decrease by cells. IAV inoculation diminished secretory degranulation and MPO secretion by neutrophils into extracellular space. It was revealed that IAV was an effective HOCl scavenger; thus, it suppressed MPO activity.
The results of the present work supposed that during IAV infection virus redox systems modified phagocytes redox systems, what displayed by the decrease of oxygen-activating ability and by the suppression of functional activity of these cells and it resulted in immunosuppressive action.

Rating:

Share or cite this content

Citations should be made in the following way:
G. Semenkova, Y. Sudnik, M. Petukh, T. Kulahava, O. Savinova (Minsk, Belarus). Modification of phagocyte redox systems by influenza A virus. Eur Respir J 2010; 36: Suppl. 54, 2991

Member's Comments

No comment yet.

Related content which might interest you: Monitoring the kinetics of a vaccine-induced T-cell response after H1N1 influenza vaccination Source: Annual Congress 2010 - Influenza A (H1N1) and other viral infections: therapeutic aspects Year: 2010 Immunological responses to influenza infection Source: Annual Congress 2006 - Influenza: from avian flu to pandemia? Year: 2006 Role of reactive persulfide species in influenza virus-induced lung inflammation in mice Source: Virtual Congress 2020 – Translational science in respiratory infections Year: 2020 Impact of the COVID-19 pandemic on Influenza virus transmission Source: Virtual Congress 2021 – COVID - 19: lessons learned Year: 2021 Emergence of an immunosuppressive neutrophil phenotype during pneumococcal lung co-infection with influenza A virus Source: International Congress 2019 – Host-microbe interactions in lung disease and exacerbations Year: 2019 Acutely lethal H1N1 influenza A virus infection alters the murine alveolar type II cell surfactant lipidome Source: International Congress 2016 – Viral infections in different respiratory conditions: from the bedside to the lab Year: 2016 Assessment of the association between asthma and immune responses to measles and mumps vaccine viruses Source: Annual Congress 2008 - Novel therapeutic approaches and immunological mechanisms in airway cell pathobiology Year: 2008 Role of influenza virus infection in lung fibrosis Source: Annual Congress 2010 - Novel cellular mechanisms in lung disease Year: 2010 LSC Abstract – The expression and function of BPIFA1 in the pulmonary innate immune response to influenza A virus infection Source: International Congress 2015 – Viral infections: basic research and clinical perspective Year: 2015 Tropism and innate host responses of influenza A/H5N6 virus: an analysis of ex vivo and in vitro cultures of the human respiratory tract Source: Eur Respir J , 49 (3) 1601710; DOI: 10.1183/13993003.01710-2016 Year: 2017 Protective efficacy of virus like particles (VLPs) containing the influenza HA and/or NA in a murine model of postinfluenza bacterial pneumonia Source: Virtual Congress 2020 – Translational science in respiratory infections Year: 2020 Possible mechanism of immunopathological damage induced by hemagglutinin protein of H5N1 Source: Annual Congress 2009 - Mechanisms of pulmonary inflammation and fibrosis Year: 2009 An innate defence role for BPIFA1/SPLUNC1 against influenza-A virus infection Source: International Congress 2015 – The multiple faces of host defense Year: 2015 Investigation of the innate antiviral response to respiratory syncytial virus Source: International Congress 2015 – Paediatric pulmonary infections: mechanisms and treatment Year: 2015 Clinical relevance of mixed respiratory viral infections in adults with influenza A H1N1 Source: Eur Respir J 2011; 38: 739-742 Year: 2011 Assessment of humoral and cell-mediated immune response to measles mumps rubella vaccine viruses among patients with asthma Source: Annual Congress 2010 - Clinical aspects of asthma and allergic respiratory diseases Year: 2010 Influenza infection of monocyte-macrophages is insensitive to inhibition by NH4Cl Source: Annual Congress 2009 - Viruses and respiratory infections: prevalence and mechanisms of infection Year: 2009 Human immune recognition-based multicomponent subunit vaccines against tuberculosis Source: Eur Respir J 2005; 25: 902-910 Year: 2005 PP230 – Functional profiling and visualization of stromal cell dynamics in the influenza virus infected lung Source: ERS Lung Science Conference 2021 Year: 2021