Source: Annual Congress 2010 - State of the art imaging

Abstract

Expression of matrix metalloproteinases (MMPs) is significantly up-regulated after cigarette smoke-induced airway inflammation. The purpose of this study was to visualize acute pulmonary inflammation through the use of real-time methods by fluorescence molecular imaging (FMI) in the lung tissue evaluating MMPs activity in response to acute cigarette smoke exposure (CSE). We exposed two mice strains, susceptible (C57BL/6j) and resistant (129S2/Sv) to develop emphysema after chronic CSE in order to characterize the profile of CSE-induced acute inflammation. We visualized in vivo pulmonary inflammation 24h after CSE by using a quantitative FMI with MMPs-2, 3, 9 and 13-sensitive activatable fluorescence probe and we examined the same MMPs protein levels in the lung tissue by Western Blot (WB). FMI analysis revealed MMPs activity increase only in susceptible mice.

This result was confirmed by measuring the MMPs protein levels in the lung tissue. Non-susceptible mice strains did not show any activity increase in MMPs activity, neither by FMI nor by WB. We demonstrate the ability to visualize and quantify in vivo pulmonary inflammation by FMI analysis after acute CSE differentiating susceptible and non-susceptible mice. These results suggest FMI could be a potential tool to follow the evolution of pulmonary inflammation with the chronic CSE leading to lung emphysema.

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L. del Puerto-Nevado, S. Pérez-Rial, A. Girón-Martínez, N. Gonzalez-Mangado, G. Peces-Barba (Madrid, Spain). Visualization of lung inflammation by fluorescence molecular imaging after acute cigarette smoke exposition in susceptible and resistant mice strains. Eur Respir J 2010; 36: Suppl. 54, 5285

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