Source: Annual Congress 2010 - COPD: treatment and monitoring
Disease area: Airway diseases

Abstract

The analysis of exhaled breath condensate (EBC) biomarkers is non-invasive method for evaluation of the oxidative stress and inflammation in the respiratory tract of patients with pulmonary diseases.
The aim: To evaluate the dynamic of pH-level, the concentration of hydrogen peroxide (H₂O₂) and nitrite (NO₂^-) in EBC from patients with COPD and asthma exacerbation before and after 14 days treatment.
Methods: We enrolled 28 patients (14 – COPD and 14 – asthma) and 14 healthy nonsmokers. EBC was collected using ECoScreen before and after 14-days treatment. pH level was measured using Beckman microelectrode. H₂O₂ concentration in EBC was detected using Prussian Blue based sensor. NO₂^- concentration in EBC was measured by modified Griess reaction. Spirometry was evaluated also.
Results: The pH was decreased and H₂O₂ was increased in EBC from patients with COPD and asthma exacerbation in compared with healthy subjects (p<0.05). NO₂^- in EBC was decreased in all groups of patients, but significant difference was only in the group of asthma compared with healthy (p=0.005). After treatment there were significant increase of pH and decrease of H₂O₂ in EBC from patients with COPD and asthma, but NO₂^- wasn‘t significant changed. Negative correlation was found between H₂O₂ and NO₂^- in EBC from asthma patients (r=-0.64). No correlations were found between dynamic of biomarkers in EBC and dynamic of lung function parameters.
Conclusion: Determination of EBC biomarkers helpS to assess inflammatory activity in the respiratory tract of patients with COPD and asthma exacerbation and effect of treatment.

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T. Anokhina, E. Anaev, A. Karyakin, V. Apyari, S. Dmitrienko, A. Chuchalin (Moscow, Russian Federation). The dynamic of pH, hydrogen peroxide and nitrite in exhaled breath condensate from patients with COPD and asthma exacerbation. Eur Respir J 2010; 36: Suppl. 54, 1330

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