Source: Annual Congress 2010 - Acute respiratory failure
Disease area: Airway diseases

Abstract

This study investigated the effects of LASSBio596, which exhibits potent inhibitory effects on TNF-α and TGF-β, associated with surfactant on lung and distal organ injury in an experimental sepsis-induced acute lung injury (ALI). 32 BALB/c mice were randomized to undergo cecal ligation and puncture surgery (CLP) or sham (C). At 1 hour, C and CLP groups were further randomized into subgroups to receive saline (0.2 ml ip), LASSBio 596 (10 mg/kg ip, 596), surfactant (Survanta® 4 ml/kg, 0.1 ml it, S) or LASSBio596 associated with surfactant (596-S). The survival rates were monitored for 7 days after CLP. At 24 hours, LASSBio596 associated or not with surfactant significantly improved survival (p<0.01) [40% (ALI) to 78% (596) and 76% (596-S)] and lung mechanics (static elastance, airway resistance, and viscoelastic pressure), reduced the amount of atelectasis, repaired epithelium and endothelium injury, attenuated lung and distal organ cell apoptosis, diminished urea, creatinine, aspartate aminotransferase and alanine aminotransferase levels, and decreased cellularity in the bronchoalveolar and peritoneal lavage fluids, and blood. Surfactant improved lung mechanics and histology but did not act on systemic inflammatory process. In conclusion, in the present ALI model, a single early dose of LASSBio596 associated or not with surfactant may modulate the inflammatory process reducing not only the risk of lung injury, but also distal organ impairment.
Supported by: INCT-INOFAR, FAPERJ, PRONEX, CNPq, CAPES

Rating:

Share or cite this content

Citations should be made in the following way:
C. Garcia, J. Silva, G. Oliveira, M. Oliveira, C. Samary, C. Araujo, G. Padilha, V. Capelozzi, L. Lima, E. Barreiro, P. Rocco (Sao Paulo, Brazil). The association between LASSBio596 and surfactant improves lung morpho-function and reduces the inflammatory process in experimental sepsis-induced acute lung injury. Eur Respir J 2010; 36: Suppl. 54, 2285

Member's Comments

No comment yet.

Related content which might interest you: Pioglitazone attenuates endotoxin-induced acute lung injury by reducing neutrophil recruitment Source: Eur Respir J 2012; 40: 416-423 Year: 2012 Effects of autophagy on LPS-induced lung inflammation and acute lung injury (ALI) in vitro and in vivo Source: International Congress 2018 – Role of microbial exposure and inflammation in lung injury Year: 2018 The effect of mercaptoethylguanidine on acute lung injury associated with sepsis Source: Eur Respir J 2003; 22: Suppl. 45, 451s Year: 2003 Mechanisms responsible for surfactant changes in sepsis-induced lung injury Source: Eur Respir J 2005; 26: 1074-1079 Year: 2005 The role of pendrin in acute lung injury caused by ventilator-induced lung injury Source: Virtual Congress 2020 – Mechanistic insight into lung injury and repair Year: 2020 Buffering of the acidosis induced by hypercapnia modulates the inflammatory process in lung and distal organs in experimental acute lung injury Source: Annual Congress 2009 - Acute lung injury: biological mechanisms Year: 2009 Ghrelin ameliorates bleomycin-induced acute lung injury by protecting alveolar epithelial cells and suppressing lung inflammation Source: Annual Congress 2012 - Fibrogenesis between epithelial injury and fibroblast proliferation Year: 2012 The effect of dexamethasone on the pulmonary fibrosis of acute lung injury induced by lipopolysaccharide in rats Source: Annual Congress 2007 - Respiratory pathophysiology Year: 2007 Therapeutic lavage with rSP-C surfactant restores oxygenation and improves histological outcome in a rat lung lavage model of acute lung injury Source: Eur Respir J 2001; 18: Suppl. 33, 7s Year: 2001 Pre-treatment with mesenchymal stem cells reduces ventilator-induced lung injury}, Source: Eur Respir J 2012; 40: 939-948 Year: 2012 The role of osteopontin in the pulmonary fibrosis caused by acute lung injury induced by lipopolysaccharide in rats Source: Annual Congress 2011 - Acute respiratory failure Year: 2011 Chronic intermittent hypoxia exacerbates lung fibrosis in bleomycin-induced lung injury mouse model Source: International Congress 2018 – Sleep-disordered breathing: cardiovascular consequences, epidemiology, phenotypes and comorbidities Year: 2018 Changes of inflammatory markers in the ventilator-induced lung injury model of rabbits Source: Eur Respir J 2004; 24: Suppl. 48, 265s Year: 2004 The role of leptin in pulmonary neutrophilia during murine acute lung injury Source: Annual Congress 2011 - Lung cell injury and repair Year: 2011 Desferrioxamine attenuates minor lung injury following surgical acute liver failure Source: Eur Respir J 2009; 33: 1429-1436 Year: 2009 Creatine supplementation attenuates systemic and pulmonary effects of acute lung injury induced by pulmonary ischemia-reperfusion in rats Source: Annual Congress 2012 - Lung transplantation: studies in candidates and recipients Year: 2012 Surfactant therapy for acute lung injury and other lung diseases Source: Eur Respir J 2003; 22: Suppl. 45, 210s Year: 2003 Phenotipic diferences between alveolar and interstitial macrophages in acute lung injury induced by pancreatitis Source: Annual Congress 2010 - Determinants of acute lung injury and fibrosis Year: 2010 Effect of sivelestat in patients with acute lung injury Source: Eur Respir J 2006; 28: Suppl. 50, 347s Year: 2006 Inflammatory responses and gas exchange in a sepsis-induced acute lung injury model Source: Eur Respir J 2005; 26: Suppl. 49, 556s Year: 2005