Source: Annual Congress 2010 - Pathology of lung cancer
Disease area: Airway diseases

Abstract

Reactive oxygen species (ROS) are important in pathogenesis of non-small cell lung cancer (NSCLC) and chronic obstructive pulmonary disease (COPD). Polymomorphonuclear neutrophils (PMN) are the main ROS producing cells. Still, an exact role of oxidative stress in pathogenesis of NSCLC, COPD and in coexistence of both diseases is unknown. The aim of the study was to evaluate PMN ROS production in NSCLC with and without COPD.
Methods. PMN were isolated by high density centrifugation from peripheral blood of 26 patients with NSCLC, 27 patients with moderate COPD, 23 patients with NSCLC and moderate COPD (NSCLC/COPD) and 16 healthy non-smoking individuals (HI). PMN suspensions (2x10⁶/ml) were incubated with ROS stimulator PMA (0.1-30 nM) for 45 min. DHR-123 was used for the detection of generated ROS. Relative ROS production was measured by fluorescence intensity in PMN population by flow cytometer.
Results. The highest ROS production was found in groups with NSCLC: NSCLC (30.9±4.8%), NSCLC/COPD (32.4±5.9 %), compared to COPD (20.4±3.7%, p<0.05) or HI (basal level). PMA stimulated a dose-dependant ROS increased in all groups. The most significant increase of ROS generation was observed at 0.1-0.3 nM PMA in NSCLC (98.9±9.6-fold) and NSCLC/COPD (92.3±10.1-fold); while in COPD and HI groups – at 0.3-1 nM PMA (82.4±11.2-fold and 43.8±8.7-fold respectively). Plateau and the highest ROS concentration was obtained at 3 nM PMA in all groups: the highest ROS was in NSCLC (221.4±17.7-fold) and NSCLC/COPD (208.1±12.3-fold), compared to COPD (173.2±11.5-fold, p<0.05) and HI (126.8±13.0-fold, p<0.05).
Conclusions. Our data suggest a higher level of ROS and PMN prestimulation both in NSCLC and NSCLC/COPD than in COPD or HI.

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N. Vaguliene, M. Zemaitis, A. Babusyte, S. Miliauskas, R. Sakalauskas (Kaunas, Lithuania). ROS production in PMN is higher during inflammation in NSCLC than in COPD. Eur Respir J 2010; 36: Suppl. 54, 3262

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