Source: Annual Congress 2010 - New treatments for asthma and COPD
Disease area: Airway diseases

Abstract

Introduction Asthma is an inflammatory disorder of airways with a spectrum of symptoms. Inflammation is thought to trigger symptoms of asthma directly and indirectly, implying that it is the primary cause of airway dysfunction in asthma. Reactive oxygen species (ROS) play an important role in inflammation in asthma. NADPH oxidase is an enzyme, responsible for ROS generation; the agent that selectively inhibits its activation is apocynin.
Aim of the study Pondering the anti-inflammatory feature of apocynin, we investigated the effect of nebulized apocynin in 10 nonsmoking asthmatics, in placebo-controlled, cross-over design study.
Methods Effects of apocynin (3 mg) have been evaluated 30, 60 and 120 minutes after nebulization by collecting exhaled breath condensate (EBC), in which we investigated H₂O₂, NO₂¯ and NO₃¯ concentrations. We also estimated safety parameters and NO₂¯ concentration in serum.
Results Apocynin decreased H₂O₂ concentration in EBC 60 min after inhalation (0.26 μM vs. 0.47 μM, p>0.05) and 120 min after inhalation (0.21 μM vs. 0.42 μM, p<0.05), comparing to placebo. Apocynin also reduced NO₂¯ concentration, 30 and 60 min after nebulization comparing to placebo (2.75 μM vs. 4.95 μM and 2.53 μM vs. 4.00 μM respectively, p<0.05). Moreover, apocynin decreased NO₃¯ concentration at each time-point (3.19 μM vs. 8.61 μM after 30 min, 5.44 μM vs. 8.63 μM after 60 min and 4.02 μM vs. 9.14 μM after 120 min respectively, p<0.05). No apocynin influence on NO₂¯ concentration in serum and safety parameters has been observed.
Conclusion Inhaled apocynin is effective and safe anti-inflammatory agent, which may be a promising compound to alleviate inflammatory process in respiratory diseases like asthma.

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Citations should be made in the following way:
J. Stefanska, A. Sarniak, A. Wlodarczyk, Z. Doniec, D. Nowak, R. Pawliczak (Rabka, Poland). Apocynin reduces reactive oxygen species generation in airways of asthmatics. Eur Respir J 2010; 36: Suppl. 54, 1159

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