Source: Annual Congress 2010 - Pathology of lung cancer

Abstract

The apoptotic volume decrease (AVD) is an early prerequisite to apoptotic events leading to cell death. We have demonstrated that AVD was associated with activity of the volume sensitive outwardly rectifying (VSOR) Cl^- channel, which is essential to the progression of apoptosis in our previous research. The present study was designed to find out whether VSOR Cl^- channel is involved in cisplatin II [cis-diaminodichloroplatinum, CDDP]-induced AVD process and cisplatin resistance of the human lung adenocarcinoma cell. The AVD process was measured in A549 cells, which serve as a model of sensitive to cisplatin and A549/CDDP cells, which serve as a model of cisplatin resistance. Whole-cell patch clamp was occupied to record VSOR Cl^- currents induced by hypotonicity. The results showed that CDDP pretreatment induced AVD process in A549 cells but not in A549/CDDP cells. The VSOR Cl^- currents were nearly absent in A549/CDDP cells compared with the parallel A549 cells. And the relative cell viability of A549/CDDP cells was much higher than A549 after treated with CDDP. Pretreatment the cells a histone deacetylase inhibitor, trichostatin A (TSA), not only partially restored VSOR Cl^- channel activity, but also decreased the cell viability of A549/CDDP cells treated with CDDP. These results indicate that impaired activity of VSOR Cl^- channel is involved in cisplatin resistance of human lung adenocarcinoma cells and restoration of the functional expression of the VSOR Cl^- channel would lead to a increase in the sensitivity of cisplatin.

Rating:

Share or cite this content

Citations should be made in the following way:
X. Min, H. Li, J. Liu, J. Wang (Beijing, China). Roles of volume-sensitive Cl^- channel in cisplatin resistance of human lung adenocarcinoma cells. Eur Respir J 2010; 36: Suppl. 54, 3272

Member's Comments

No comment yet.

Related content which might interest you: The role of NHE-1 on multidrug-resistance mechanisms of human small cell lung cancer cells Source: Eur Respir J 2007; 30: Suppl. 51, 537s Year: 2007 Relationship between mutated P53 and the acquired multi-drug resistance after cisplatin selection in NCI-H460 cell line of human large cell lung cancer Source: Annual Congress 2004 - Molecular biology Year: 2004 Adaptation to hypoxia leads to apoptosis resistance to cisplatin in non-small cell lung cancer (NSCLC) cell line Source: Annual Congress 2008 - Biology and prognosis in thoracic oncology Year: 2008 Dihydroartemisinin combined with cisplatinum induced apoptosis and related mechanism research in human lung adenocarcinoma H1299 cells Source: International Congress 2016 – Lung cancer and immune cells: friends or foes? Year: 2016 Inhibition of autophagy potentiates pemetrexed and simvastatin-induced apoptotic cell death in malignant mesothelioma and non-small cell lung cancer cells Source: International Congress 2015 – Cell biology of lung cancer and new biomarkers Year: 2015 HIF-1α modulation by topoisomerase inhibitors in A549, a non-small cell lung cancer cell line Source: Annual Congress 2007 - Biology in thoracic oncology Year: 2007 Deguelin enhances chemosensitivity of non-small cell lung cancer cells Source: Eur Respir J 2006; 28: Suppl. 50, 336s Year: 2006 Over-expression of aquaporin-5 increases migration and metastatic potential of lung cancer cells Source: Annual Congress 2009 - Hot topics in molecular pathology and functional genomics of lung cancer Year: 2009 A possible role of intracellular calcium in the development of anti-cancer therapy -resistance in lung cancer cells Source: Eur Respir J 2006; 28: Suppl. 50, 853s Year: 2006 Autophagy induces cytoprotection in cisplatin-treated lung cancer cells Source: Annual Congress 2010 - Pathology of lung cancer Year: 2010 Prx1 provides resistance to docetaxel-induced apoptosis in lung cancer cells Source: Annual Congress 2010 - Prognostic factors for lung cancer Year: 2010 High CO2 tumor microenvironment confers chemoresistance in lung cancer cells. Source: International Congress 2017 – Lung cancer: latest basic research on molecular alterations, tumour-immune system-microenvironment interplay and the metabolome Year: 2017 Prx1 modulates the chemosensitivity of lung cancer to docetaxel through suppression of FOXO1-induced apoptosis Source: Annual Congress 2012 - New insights in the pathology of lung cancer Year: 2012 ABCG2 is determinant to SN-38 resistance in non-small cell lung cancer Source: Annual Congress 2005 - Prognostic factors in lung cancer Year: 2005 Putative cancer stem cells in malignant pleural mesothelioma show resistance to cisplatin and pemetrexed Source: Annual Congress 2010 - Recent developments of molecular pathology and functional genomics in non-neoplastic and neoplastic lung diseases Year: 2010 Two-pore domain K+ channel TASK-1 maintains the membrane potential in non-small cell lung cancer (NSCLC) cells Source: Annual Congress 2008 - Biology and diagnosis of thoracic tumours Year: 2008 Bile acid receptor mediates cancer-associated fibroblasts-induced migration and invasion of non-small cell lung cancer cells Source: International Congress 2017 – Basic research in lung cancer: a scientific potpourri from genetic alterations to immune cells Year: 2017 TGFß1-induces resistance to apoptosis in human lung myofibroblasts via TRPA1 downregulation Source: International Congress 2019 – Translational aspects of idiopathic pulmonary fibrosis Year: 2019 p53 mutations do not predict response to paclitaxel in metastatic non-small cell lung carcinoma Source: Annual Congress 2009 - Lung cancer biology Year: 2009 Shikonin-induced necroptosis is enhanced by the inhibition of autophagy in non-small cell lung cancer cells Source: International Congress 2016 – Basic science and case reports in lung cancer Year: 2016