Source: Annual Congress 2010 - Influenza A (H1N1) and other viral infections: therapeutic aspects
Disease area: Respiratory infections

Abstract

Introduction
The entry of major type rhinovirus, type 14 rhinovirs (RV14) into the cytoplasm of infected cells is mediated by the destabilization from receptor binding, by endosomal acidification, or both. Proton pump inhibitors (PPIs), the inhibitors on H-K ATPase in gastric parietal cells, are related to these mechanism. Therefore, it is expected to inhibit infection.
Methods
Human tracheal epithelial cells were cultured and was infected with the RV14 (5 log TCID50 units/ml) . Cells were treated with lansoprazole (10 μM) before and after the infection and used to examine the inhibitory effects.
Results
Lansoprazole significantly decreased the viral titers of the supernatants at day 1 (4.1±0.1 log TCID50 units/ml vs. 3.0±0.3 log TCID50 units/ml, n=5) and day 3 (4.7±0.2 log TCID50 units/ml vs. 3.3±0.3 log TCID50 units/ml, n=5). Lansoprazole also inhibited the ICAM-1 mRNA expression in the human cultured tracheal epithelial cells (0.69±0.11 ICAM-1/GAPDH ratio compared with control, n=3), and reduced the contents of soluble form of ICAM-1 in the supernatants (205±15 ng/ml vs. 148±8 ng/ml, n=3) before RV14 infection. Lansoprazole decreased the number of acidic endosomes and the fluorescence intensity from the acidic endosomes in the epithelial cells.
Conclusions
These findings suggest that lansoprazole may inhibit RV14 infection by reducing ICAM-1 and by blocking the rhinovirus RNA entry into the endosomes.

Rating:

Share or cite this content

Citations should be made in the following way:
T. Sasaki, H. Yasuda, D. Inoue, T. Nukiwa, M. Yamaya (Sendai, Japan). The proton pump inhibitor, lansoprazole, inhibits rhinovirus infection in the cultured human tracheal epithelial cells. Eur Respir J 2010; 36: Suppl. 54, 2988

Member's Comments

No comment yet.

Related content which might interest you: An inhibitor of HMG-CoA reductase, pitavastatin, inhibits rhinovirus infection in human tracheal epithelial cells Source: Annual Congress 2009 - Lung cell biology and immunology Year: 2009 Carbocisteine inhibits rhinovirus infection in human tracheal epithelial cells Source: Eur Respir J 2006; 28: 51-58 Year: 2006 Effect of roflumilast, a PDE4 inhibitor, on ciliary beat frequency in human nasal epithelial cells Source: Annual Congress 2008 - Novel experimental approaches to understanding asthma and COPD Year: 2008 Effect of clarithromycin on rhinovirus-16 infection in A549 cells Source: Eur Respir J 2006; 27: 12-19 Year: 2006 Protective effect of MAPK inhibitors and budesonide against apoptosis induced by TGF-β in primary cultures of human bronchial epithelial cells Source: Eur Respir J 2003; 22: Suppl. 45, 394s Year: 2003 Effects of the PDE4 inhibitor roflumilast n-oxide on the adherence of polymorphonuclear cells (PMNC) to bronchial epithelial cells (BEAS-2B) induced by non-typeable Haemophilus influenzae Source: Annual Congress 2009 - Highlighting the epithelial cell in lung disease: from COPD to cancer to acute lung injury Year: 2009 Roflumilast N-oxide (RNO) alleviates from RSV-induced loss of ciliated cells and enhanced MUC5AC in differentiated human bronchial epithelial cells (D-HBE) Source: Annual Congress 2010 - Regulation of airway pharmacology Year: 2010 The PDE4 inhibitor roflumilast N-oxide partly restored cigarette smoke (CS)-induced tight junction disassembly in human airway epithelial cells (Calu-3) – role of MAPK Source: Annual Congress 2008 - Basic science in asthma and COPD II Year: 2008 Physiomer(R) reduces the chemokine interleukin-;8 production by activated human respiratory epithelial cells Source: Eur Respir J 2001; 18: 661-666 Year: 2001 Induction of apoptosis in murine alveolar epithelial cells by a proteasome inhibitor Source: Eur Respir J 2001; 18: Suppl. 33, 358s Year: 2001 Human airway trypsin-like protease stimulates differentiation of fibroblasts to myofibroblasts in human bronchial fibroblasts in vitro Source: International Congress 2018 – Identification of therapeutic targets for asthma Year: 2018 Simvastatin selectively inhibits TSLP-production in primary bronchial epithelial cells from COPD donors Source: Annual Congress 2011 - Translational models of disease Year: 2011 Effect of roflumilast N-oxide on non-typable haemophilus influenzae (NTHi) interaction with human airway epithelial A549 cells Source: Annual Congress 2011 - Epithelial cell biology Year: 2011 PUR1800 (RV1162), A novel narrow spectrum kinase inhibitor, but not Fluticasone, reduces TNFa-induced cytokine release by primary bronchial epithelial cells from healthy volunteers and COPD patients Source: International Congress 2018 – Preclinical pharmacology: novel targets in airway inflammation, narrowing and neural control Year: 2018 The PDE4 inhibitor roflumilast N-oxide partly reversed TGFß1-induced changes in collagen I and E-cadherin expression in human airway epithelial cells Source: Annual Congress 2008 - Novel experimental approaches to understanding asthma and COPD Year: 2008 Adenovirus E1A suppressed elafin secretion in human bronchial epithelial cells and alveolar epithelial cells Source: Eur Respir J 2004; 24: Suppl. 48, 554s Year: 2004 Inhibition of Pim1 kinase reduces viral replication in primary bronchial epithelial cells Source: Eur Respir J 2015; 45: 1745-1748 Year: 2015 LATE-BREAKING ABSTRACT: Effects of a single treatment with PC786, a novel inhibitor of respiratory syncytial virus replication, on viral load and biomarkers in fully differentiated human bronchial epithelial cells Source: International Congress 2016 – Translational research in respiratory infections Year: 2016 Inhibitory effects of tiotropium on rhinovirus infection in human airway epithelial cells Source: Eur Respir J 2012; 40: 122-132 Year: 2012 Adenoviral infection does not increase β-defensin1-3 and RNAse7 mRNA levels in A549 cells and respiratory epithelial cell cultures derived from human adenoids and human bronchial biopsies Source: Eur Respir J 2006; 28: Suppl. 50, 563s Year: 2006