Source: Annual Congress 2010 - Acute respiratory failure
Disease area: Respiratory critical care

Abstract

Background: Vaporized perfluorocarbon (PFC) has been used for treatment of lung injury. However, its potential mechanism is still unclear. Furthermore, it has not been determined whether PFC has a protective effect on other organs. Methods: Forty female New Zealand rabbits were ventilated in a volume-controlled anesthetic machine. Acute lung injury (ALI) was induced by lung lavage with artificial seawater. The animals (n=8 in each group) received conventional mechanical ventilation (CMV), positive end-expiratory pressure (PEEP), PFC or PEEP+PFC. The lung, small intestine, liver and kidney were harvested at the end of the experiments and assayed by histopathological techniques. The expression of matrix metalloproteinase (MMP)-9 in each lung was determined by real-time PCR.Results: Pathological injury of the lung was significantly alleviated (P < 0.01) in the PEEP, PFC, and PFC+PEEP groups, but injury to the liver was significantly alleviated in the PEEP group. Although there was a tendency for renal and small intestine injury to be alleviated in the PEEP, PFC, and PFC+PEEP groups, these differences were not significant (P > 0.05). The expression of MMP-9 mRNA in the PEEP, PFC and PFC+PEEP groups was significantly lower than in the CMV group (P < 0.01). The expression of MMP-9 was lowest in the PFC+PEEP groups and was significantly lower than that in the PEEP group (P < 0.05). Conclusions: Vaporized PFC can significantly improve oxygenation and pulmonary function but has no significant protective effect on other organs. The mechanism of alleviating lung injury might be associated with the inhibition of MMP-9 expression.

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Citations should be made in the following way:
B. Han, X. Zhao, X. Huang, L. Xie (Beijing, China). Vaporized perfluorocarbon confers protection against acute lung injury by inhibiting MMP-9 expression without protective effects in other organs. Eur Respir J 2010; 36: Suppl. 54, 2287

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