Source: Annual Congress 2010 - Pathology of lung cancer

Abstract

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Cislplatin-based chemotherapy is main regimen for advanced lung cancer. But serious adverse reaction had occurred after chemotherapy. This is main reason for chemotherapy withdrawal. To whether the effect of low dose of cisplatin will be synergistically enhance by autophagy inhibiton, we investigated that role of auto- phagy by 3-methyladenine in cisplatin-treated lung cancer cells. Lung cancer cells were incubated with normal media and treated the 5uM and 20uM of cislpaltin according to time dependent. We examined the sensitivity to low or high doses of cisplatin treatment and compared apoptosis and autophagy by nuclear stianing, apoptotic or autophagic related proteins or autophagic vacuoles. autophagy is also displayed the development of acidic vascular organelles by acridine orange and the fluorescent expression of GFP-LC3 protein in the GFP-LC3 transfected cells. Low dose of cisplatin-treated lung cancer cells is relatively less sensitive to cell death rather than high dose treated cells in a time-dependent manners. We observed that massive vacuolization in the cytoplasm were seen at lower dose treated cells. Acridine orange stain-positive cells also were increased according to time depen- dent. The autophagosome-incorporated LC3 II protein expression more increased in low dose treated cells in spite of no detection of LC3 II in high dose treated cells. The expression of GFP-LC3 were increased in 5uM treated cells by time-depen dent manner. When 3-methyladenine, was pretreated in cisplatin-treated lung cancer cells, cell survival significantly were decreased. Autophagy induces cytoprotection in lower dose of cisplatin-treated lung cancer cells.

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S. H. Yang, H. Y. Jang, H. J. Jo, K. K. Lee, S. R. Moon, S. A Park, S. Y. Lee (Iksan, Republic Of Korea). Autophagy induces cytoprotection in cisplatin-treated lung cancer cells. Eur Respir J 2010; 36: Suppl. 54, 3261

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