Source: Annual Congress 2005 - Skeletal muscles: current concepts
Disease area: Airway diseases

Abstract

Increased protein carbonylation has been shown in quadriceps of COPD patients. Our aim was to assess whether creatine kinase (CK) is carbonylated and whether the degree of carbonylation influences its activity in quadriceps of COPD patients. Methods: Quadriceps biopsies were obtained from 6 normal subjects and 12 COPD patients. Protein carbonylation intensity and identification of carbonylated proteins was achieved using the Oxyblot kit, 2D electrophoresis, and Mass Spectrometry analysis. CK expression and activity were also measured. Results: CK protein expression and activity were significantly higher in quadriceps of COPD patients compared with controls. Both muscle CK and carbonic anyhdrase III (CAIII) were the most intensely carbonylated proteins in both controls and COPD patients. Within COPD patients, muscle CK protein levels and activity positively correlated with FEV1 (% pred.) (r=0.82,** and r=0.66,*, respectively), while muscle CK carbonylation intensity did it negatively (r=0.88, *). Furthermore, CK activity negatively correlated with muscle CK carbonylation intensity. Conclusions: Peripheral muscles of COPD patients undergo adaptation by increasing CK protein expression and activity, and both CK and CAIII appear to be important targets for protein carbonylation in those muscles.
Funded by: Enigma in COPD QLRT-2002-02285 (EU), RESPIRA (RTIC C03/11).

Rating:

Share or cite this content

Citations should be made in the following way:
E. Barreiro, J. Gea, E. Melo, S. N. Hussain (Barcelona, Spain; Montreal, Canada). Creatine kinase enzyme is carbonylated in the quadriceps of COPD patients. Eur Respir J 2005; 26: Suppl. 49, 1668

Member's Comments

No comment yet.

Related content which might interest you: Protein oxidation in the diaphragm of patients with COPD Source: Eur Respir J 2003; 22: Suppl. 45, 358s Year: 2003 Lactate dehydrogenase isozymes in skeletal muscle of COPD patients Source: Eur Respir J 2006; 28: Suppl. 50, 160s Year: 2006 Pathways associated with reduced quadriceps oxidative fibres and endurance in COPD Source: Eur Respir J 2013; 41: 1275-1283 Year: 2013 Metabolic characteristics of the deltoid muscle in patients with chronic obstructive pulmonary disease Source: Eur Respir J 2001; 17: 939-945 Year: 2001 Glutamate and glutathione levels are preserved in the diaphragm of patients with COPD Source: Eur Respir J 2002; 20: Suppl. 38, 18s Year: 2002 Effects of exercise on ubiquitination and proteasome activity in skeletal muscle in COPD patients Source: Annual Congress 2012 - Exercise testing in disease Year: 2012 Association between creatine kinase level and mortality in high-risk patients with COVID-19 Source: Virtual Congress 2021 – COVID - 19 biomarkers Year: 2021 Expression and functional significance of nicotinamide N-methyltransferase (NNMT) in quadriceps and diaphragm of patients with COPD Source: Annual Congress 2008 - Respiratory muscles in disease Year: 2008 Uncoupling protein 3 content is decreased in skeletal muscle of patients with COPD Source: Eur Respir J 2002; 20: Suppl. 38, 88s Year: 2002 Inactivation of glycogen synthase kinase 3β (GSK-3β) enhances skeletal muscle oxidative metabolism Source: International Congress 2015 – Genetics and environmental factors in phenotypes of asthma and COPD Year: 2015 Reduced mitochondrial density in the quadriceps muscle of patients with COPD Source: Eur Respir J 2006; 28: Suppl. 50, 621s Year: 2006 Quadriceps myostatin expression in COPD Source: Eur Respir J 2010; 36: 686-688 Year: 2010 Validation of AMP-activated protein kinase as a therapeutic target in bronchiectasis Source: ERS Lung Science Conference 2020 Year: 2020 Increased phosphorylated p38 mitogen-activated protein kinase in COPD lungs Source: Eur Respir J 2013; 42: 28-41 Year: 2013 Metabolic stress during exercise in the skeletal muscles of COPD patients Source: Annual Congress 2003 - Exercise in COPD Year: 2003 Inactivation of glycogen synthase kinase-3 is not required for recovery of muscle mass of disuse-atrophied muscle Source: International Congress 2015 – New insights into exercise and muscle performance Year: 2015 NF-κB and AP-1 activation in the quadriceps of COPD patients Source: Annual Congress 2010 - Interaction between respiratory and peripheral muscles Year: 2010 Impaired skeletal muscle kynurenine metabolism in patients with COPD Source: International Congress 2018 – Functional genomics and COPD: new challenges Year: 2018 Vastus lateralis proteomic analysis in muscle wasted patients with COPD Source: Annual Congress 2013 –The best abstracts in physical inactivity and exercise training in COPD Year: 2013 Antioxidant enzyme increase following magnetic stimulation training in the quadriceps of patients with severe COPD Source: Eur Respir J 2007; 30: Suppl. 51, 727s Year: 2007