Source: Annual Congress 2008 - Genetics, immunology and microbiology of tuberculosis
Disease area: Respiratory infections



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Citations should be made in the following way:
A. Safianowska, R. Walkiewicz, P. Nejman-Gryz, R. Chazan, H. Grubek-Jaworska (Warsaw, Poland). Comparison of DNA strip assay and high performance liquid chromatography (HPLC) analysis of mycolic acids for identification of mycobacterial isolates to the species level. Eur Respir J 2008; 32: Suppl. 52, 4528

Member's Comments

Aleksandra Safianowska - 08.10.2008 08:31
Limitation of the technique
Thank you for the question. Yes, there is a space limitation on a strip, where the DNA probes are placed. That is why two kits have been developed, CM and AS, to be done one after onother from the same PCR amplicon. And the secon limitation, more serious, is the limitted number of specific DNA probes. The techniques, that is easy to perform and not time consuming, has been developed to be implemented into routine laboratory work flow in order to type the most common mycobacteria.

Paul Kelly - 07.10.2008 10:26
P Kelly
With now over 120 species or types of non tuberculous mycobacteria described in addition to TB (human, bovis etc) and more mycobacteria being implicated in disease is there a numerical limit to the number of species which can be detected by the technique ?

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