An RNA-seq primer for pulmonologists

Sarah G. Chu, Sergio Poli De Frias, Benjamin A. Raby, Ivan O. Rosas

Source: Eur Respir J, 55 (1) 1801625; 10.1183/13993003.01625-2018
Journal Issue: January
Disease area: -

Congress or journal article abstractFull text journal articlePDF journal article, handout or slides

Rating: 0
You must login to grade this presentation.

Share or cite this content

Citations should be made in the following way:
Sarah G. Chu, Sergio Poli De Frias, Benjamin A. Raby, Ivan O. Rosas. An RNA-seq primer for pulmonologists. Eur Respir J, 55 (1) 1801625; 10.1183/13993003.01625-2018

You must login to share this Presentation/Article on Twitter, Facebook, LinkedIn or by email.

Member's Comments

No comment yet.
You must Login to comment this presentation.


Related content which might interest you:
Molecular detection of lung cancer using common-MAGE primer and -SSX primer
Source: Eur Respir J 2004; 24: Suppl. 48, 79s
Year: 2004

Transcriptome sequencing (RNA-Seq) of human endobronchial biopsies: asthma versus controls
Source: Eur Respir J 2013; 42: 662-670
Year: 2013



Microdissection and cDNA arrays can be combined by RNA preamplification for a cell-type specific expression profiling
Source: Eur Respir J 2001; 18: Suppl. 33, 161s
Year: 2001

Detection of rifampin resistance mutations in Mycobacterium tuberculosis by use of sequencing, molecular beacons and taqman probes
Source: Annual Congress 2009 - Diagnostic advances in tuberculosis
Year: 2009


Practical approaches to analysis of DNA microarrays
Source: Annual Congress 2004 - PG7 - Application of genomics and proteomics to pulmonary disease
Year: 2004


Bacterological assessment of healthcare-associated pneumonia (HCAP) using 16S rRNA gene sequencing analysis
Source: Annual Congress 2012 - Epidemiology: risk factors and prognosis in respiratory infections
Year: 2012


Oligonucleotide array analysis (DNA-chip) of gene expression in skeletal muscle of COPD
Source: Eur Respir J 2002; 20: Suppl. 38, 496s
Year: 2002

Suitable reference genes determination for real-time PCR using induced sputum samples
Source: Eur Respir J, 54 (6) 1800644; 10.1183/13993003.00644-2018
Year: 2019



Quantitative PCR analysis of fungal DNA in European classrooms and association with respiratory health and pulmonary function in children (HESE study)
Source: Annual Congress 2010 - Genetics and risk factors for asthma and COPD
Year: 2010

Diagnostic value of polymerase chain reaction (PCR) in the management of pediatric tuberculosis
Source: Eur Respir J 2002; 20: Suppl. 38, 581s
Year: 2002

Appropriately timed COVID-19 PCR testing for hospital inpatients.
Source: Virtual Congress 2021 – Diagnosis and management of COVID-19
Year: 2021


Serum microRNA signatures identified by Solexa sequencing predict sepsis patients mortality: A prospective observational study
Source: Annual Congress 2012 - Infections and sepsis in the ICU
Year: 2012

Detection of isoniazid resistance mutations in Mycobacterium tuberculosis by use of sequencing, PCR-RFLP and TaqMan probes
Source: Annual Congress 2010 - Tuberculosis drug resistance
Year: 2010

A new integrated PCR and microarray lab-on-chip for rapid MDR tuberculosis diagnosis
Source: Annual Congress 2011 - Tuberculosis: from bench to bedside
Year: 2011

Profiling the COPD airway microbiome using quantitative culture and 16S rRNA gene sequencing
Source: International Congress 2016 – Clinical and microbiological aspects of infections in COPD patients
Year: 2016


Multiplex polymerase chain reaction - An innovative clinical tool
Source: International Congress 2015 – Promising novel findings in translational pulmonary research
Year: 2015

An automated nested multiplex PCR system to detect 16 respiratory viruses
Source: Eur Respir J 2007; 30: Suppl. 51, 406s
Year: 2007

Techniques: culture, identification and 16S rRNA gene sequencing
Source: Eur Respir Monogr 2019; 83: 18-34
Year: 2019


Microfluidics and HPLC analyses of novel lung cancer genes identified by arbitrarily primed PCR
Source: Eur Respir J 2005; 26: Suppl. 49, 456s
Year: 2005