Background : Dust mites have been considered to be the most important allergens in China, while pollen allergens are often neglected, especially in southern China. We tried to use allergen microarray to investigate the distribution of pollen allergens in southern China.Methods: The study was conducted using patient sera from Guangzhou. In total 60 sera with one or more sIgE-positive were selected for testing against 112 individual allergens. Timothy and Bermuda grass extract specific IgE were also detected by ImmunoCAP.Results: In 60 selected patients, 33.3% patients showed IgE-reactivity towards pollen allergen. 18.3% were IgE positive to nCyn d 1 (bermuda grass), 16.7% to rPhl p 4 (timothy grass). 11.7% to rPla a 2 (plane tree), 10.0% to nCup a 1 (cypress), 5.0% to nCry j 1 (Japanese cedar) and 3.3% positive to nArt v 1 (mugwort) and nSal k 1 (saltwort). There were 8.3% with nMUXF3 sIgE positive, the CCD marker. The distribution in these patients were shown in

.Conclusions: This study clearly confirms that grass group 1 are major allergens of grass pollen allergens. With the urbanization and living environment changing, specific IgE-sensitization towards pollen allergens is increasing in southern China.These pollen allergy patients are major multi-sensitized. There may be cross-reactivitis between the pollen allergens, causing by nMUXF3. Extracts and components showed simultaneously positive detected by ImmunoCAP and ISAC.