Suitable reference genes determination for real-time PCR using induced sputum samples

Catherine Moermans, Esteban Deliege, Dimitri Pirottin, Christophe Poulet, Julien Guiot, Monique Henket, Jane da Silva, Renaud Louis

Source: Eur Respir J, 54 (6) 1800644; 10.1183/13993003.00644-2018
Journal Issue: December
Disease area: Airway diseases

Congress or journal article abstractFull text journal articlePDF journal article, handout or slides

Rating: 0
You must login to grade this presentation.

Share or cite this content

Citations should be made in the following way:
Catherine Moermans, Esteban Deliege, Dimitri Pirottin, Christophe Poulet, Julien Guiot, Monique Henket, Jane da Silva, Renaud Louis. Suitable reference genes determination for real-time PCR using induced sputum samples. Eur Respir J, 54 (6) 1800644; 10.1183/13993003.00644-2018

You must login to share this Presentation/Article on Twitter, Facebook, LinkedIn or by email.

Member's Comments

No comment yet.
You must Login to comment this presentation.


Related content which might interest you:
Rapid detection of M.tuberculosis drug resistance to second line antibiotics in sputum samples by using the multi-competitive allele-specific real-time PCR
Source: Annual Congress 2010 - Scientific advances in tuberculosis
Year: 2010

Identification of stable housekeeping genes for real-time PCR in human pulmonary fibroblasts
Source: Annual Congress 2011 - Lung development and neoplasia
Year: 2011


Sensitive and specific detection of lung cancer DNA in bronchial washing by a novel methylation-specific droplet digital PCR
Source: International Congress 2017 – Clinical problems in lung diseases
Year: 2017


Rapid detection of M. tuberculosis gene mutations associated with drug resistance by modified allele-specific real-time PCR
Source: Eur Respir J 2006; 28: Suppl. 50, 589s
Year: 2006

Rapid diagnostic methods: from urinary antigens to multiplex PCR
Source: Annual Congress 2008 - Is microbiological diagnosis of community-acquired pneumonia possible and useful?
Year: 2008


Rapid DNA extraction from dried blood spots for the alpha-1 antitrypsin deficiency detection by real-time PCR
Source: Annual Congress 2012 - Recent progress in mechanisms and diagnostics of COPD and asthma
Year: 2012


LATE-BREAKING ABSTRACT: Selection of suitable housekeeping genes for real-time quantitative PCR in CD4+ lymphocytes from asthmatics with or without depression
Source: Annual Congress 2012 - Recent progress in mechanisms and diagnostics of COPD and asthma
Year: 2012

The detection of EGFR mutations in liquid biopsy by allele-specific qPCR – the comparative evaluation of two common diagnostic tests
Source: International Congress 2018 – Genetics in chronic pulmonary diseases
Year: 2018

Use of a multiplex real-time PCR for differential diagnosis of respiratory viral infections
Source: Annual Congress 2009 - Viruses and respiratory infections: prevalence and mechanisms of infection
Year: 2009


Microfluidics and HPLC analyses of novel lung cancer genes identified by arbitrarily primed PCR
Source: Eur Respir J 2005; 26: Suppl. 49, 456s
Year: 2005

Diagnostic performance of multiplex bacterial PCR in BAL for bacterial pneumonia varies across molecular assays
Source: Virtual Congress 2020 – Biomarkers and characterisation of immune response in respiratory infection
Year: 2020


Detection of Mycobacterium tuberculosis in sputum samples using polymerase chain reaction (PCR)
Source: Eur Respir J 2001; 18: Suppl. 33, 496s
Year: 2001

Evaluation of PCR for the rapid diagnosis of TBC pleuritis. Detection of two different DNA sequences
Source: Eur Respir J 2002; 20: Suppl. 38, 580s
Year: 2002

An automated nested multiplex PCR system to detect 16 respiratory viruses
Source: Eur Respir J 2007; 30: Suppl. 51, 406s
Year: 2007

Demonstration of chlamydia species in induced sputum from COPD patients by nested PCR and immunofluorescence
Source: Eur Respir J 2003; 22: Suppl. 45, 351s
Year: 2003

Development of real-time PCR technique for detection of the M.tuberculosis DNA mutations associated with fluoroquinolones drug resistance
Source: Annual Congress 2008 - The challenge of multidrug-resistant tuberculosis
Year: 2008

PCR is a sensitive and specific method for the laboratory diagnosis of mycoplasma pneumonia
Source: Eur Respir J 2002; 20: Suppl. 38, 220s
Year: 2002

Increased sputum IL-1β and IL-5 gene expression in exacerbations of COPD demonstrated with real-time PCR
Source: Eur Respir J 2004; 24: Suppl. 48, 319s
Year: 2004

Is polymerase chain reaction (PCR) an alternative for broncoscopy in patients with sputum smear negative pulmonary tuberculosis?
Source: Eur Respir J 2004; 24: Suppl. 48, 425s
Year: 2004

Selection of housekeeping genes for real-time PCR in atopic human bronchial epithelial cells
Source: Eur Respir J 2008; 32: 755-762
Year: 2008