Abstract
Background: Grape seed proanthocyanidin extract (GSPE) has more powerful antioxidative activity than other well-known antioxidants, including vitamin C and E. Idiopathic pulmonary fibrosis is a chronic progressive disorder with a poor prognosis. An oxidant-antioxidant imbalance may contribute to the disease process in idiopathic pulmonary fibrosis.
Objectives: To examine whether GSPE which is known to act as an antioxidant has therapeutic effect on bleomycin-induced pulmonary fibrosis in mice, an animal model of idiopathic pulmonary fibrosis.
Methods: Mice were treated by intratracheal instillation of bleomycin. GSPE was administered by intraperitoneal (IP) injections (30, 60, or 90 mg/kg).
Mice were sacrificed on days 21 after bleomycin instillation.
Results: Compared with the BLM/Veh group, histologic findings in mice treated with BLM and IP injection of GSPE (BLM/GSPE) showed less fibrotic lesions in a dose-dependent manner. The mean Aschcroft‘s fibrosis score in the BLM/Veh group was significantly higher than in the BLM/GSPE group.
The lung hydroxyproline concentration on Day 21 was significantly higher in the BLM/Veh group than in the BLM/GSPE group (62.9 vs 55.3, 51.9, 50.4 ug/ml).
Conclusions: GSPE attenuated bleomycin-induced pulmonary fibrosis in mice, suggesting that GSPE may be useful in the treatment of idiopathic pulmonary fibrosis.